Polasek J, Richardson M, Moore M A, Blajchman M A
Thromb Res. 1987 Mar 15;45(6):771-82. doi: 10.1016/0049-3848(87)90087-9.
Stimulated platelets release the content of their granules to the environment by a process known as platelet secretion. The precise mechanism of platelet secretion is poorly understood. The most widely-held theory suggests that, during platelet activation, secretory granules centralize and their content is released into the surface-connected canalicular system. Using fixation techniques directed at preserving membrane structures, morphological evidence is provided that human platelet activation is associated with secretory granules migrating to the periphery of platelets, where they undergo transition to participate in the formation of membrane-associated multivesicular structures. These multivesicular structures then undergo dissolution resulting in granule content secretion to the environment. The formation of the membrane-associated multivesicular structures occurs in platelets in which some of the secretory granules have also become centralized. This suggests that during the activation of human platelets, membrane-associated multivesicular structure release may occur concurrently with other mechanisms of secretory granule content release. The present observations are consistent, however, with the hypothesis that during human platelet activation the formation of membrane-associated multivesicular structures by platelet secretory granules contributes importantly to the mechanism of platelet secretion.
受刺激的血小板通过一种称为血小板分泌的过程将其颗粒内容物释放到周围环境中。血小板分泌的确切机制尚不清楚。目前最广泛认可的理论认为，在血小板活化过程中，分泌颗粒集中，其内容物释放到与表面相连的小管系统中。使用旨在保存膜结构的固定技术，提供了形态学证据，表明人类血小板活化与分泌颗粒迁移到血小板周边有关，在那里它们经历转变以参与膜相关多囊泡结构的形成。然后这些多囊泡结构发生溶解，导致颗粒内容物分泌到周围环境中。膜相关多囊泡结构的形成发生在一些分泌颗粒也已集中的血小板中。这表明在人类血小板活化过程中，膜相关多囊泡结构的释放可能与分泌颗粒内容物释放的其他机制同时发生。然而，目前的观察结果与以下假设一致，即在人类血小板活化过程中，血小板分泌颗粒形成膜相关多囊泡结构对血小板分泌机制有重要贡献。
