Wu Xin, Wei Xuan, Li Xiaofeng, Deng Jiehua, Zhang Jianquan
Department of Respiratory and Critical Medicine, The First Affiliated Hospital of Guangxi Medical University, China.
Department of Respiratory Medicine, The Forth Affiliated Hospital of Guangxi Medical University, China.
Jpn J Infect Dis. 2022 Nov 22;75(6):560-568. doi: 10.7883/yoken.JJID.2022.153. Epub 2022 Jul 29.
Dynamic changes in the microbiome during chronic obstructive pulmonary disease (COPD) exacerbations remain unclear. Using 16S ribosomal DNA and fungal internal transcribed spacer DNA sequencing, we described the composition and changes in the bacterial and fungal microbiota of bronchoalveolar lavage fluid samples from 15 COPD patients and seven non-COPD patients. In patients with COPD, the dominant bacterial phyla were Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria. The most abundant fungal phyla were the Ascomycota and Basidiomycota. In terms of the genera of bacteria and fungi, the numbers of Veillonella, Prevotella-7, Haemophilus, and Aspergillus were significantly higher in patients with COPD than in the non-COPD patients. In addition, after the progression of COPD, the relative abundances of the fungal genera Aspergillus, Mortierella, Grifola, Thermoascus, Russula, and Thermomyces and the bacterial genus Proteobacteria increased significantly. Existing analyses have demonstrated changes in the diversity of bacterial and fungal communities, which appear to be related to COPD. Our results demonstrate the potential utility of microbiota as a possible biomarker for disease progression and provide therapeutic targets for COPD.
慢性阻塞性肺疾病（COPD）急性加重期微生物群的动态变化仍不清楚。我们使用16S核糖体DNA和真菌内转录间隔区DNA测序，描述了15例COPD患者和7例非COPD患者支气管肺泡灌洗液样本中细菌和真菌微生物群的组成及变化。在COPD患者中，主要的细菌门类为变形菌门、厚壁菌门、拟杆菌门和放线菌门。最丰富的真菌门类是子囊菌门和担子菌门。在细菌和真菌属方面，COPD患者中韦荣球菌属、普雷沃菌属7、嗜血杆菌属和曲霉属的数量显著高于非COPD患者。此外，COPD进展后，真菌属曲霉属、被孢霉属、猪苓属、嗜热子囊菌属、红菇属和嗜热真菌属以及细菌属变形菌门的相对丰度显著增加。现有分析表明细菌和真菌群落的多样性发生了变化，这似乎与COPD有关。我们的结果证明了微生物群作为疾病进展可能的生物标志物的潜在用途，并为COPD提供了治疗靶点。
