Schwarz Luisa Vivian, Valera Maria Jose, Delamare Ana Paula Longaray, Carrau Francisco, Echeverrigaray Sergio
University of Caxias do Sul (UCS), Institute of Biotechnology, Francisco Getúlio Vargas 1130, 95070-560 Caxias do Sul, RS, Brazil.
Enology and Fermentation Biotechnology Area, Departamento Ciencia y Tecnología Alimentos, Facultad de Química, Universidad de la Republica de Uruguay, Montevideo, Uruguay.
Curr Res Microb Sci. 2022 Mar 26;3:100129. doi: 10.1016/j.crmicr.2022.100129. eCollection 2022.
Yeasts of the genus gained notoriety in the last years due to their contribution to wine quality, and their loss of several genes, mainly related to DNA repair and cell cycle processes. Based on genomic data from many members of this genus, they have been classified in two well defined clades: the "faster-evolving linage" (FEL) and the "slower-evolving lineage" (SEL). In this context, we had detected that exhibited a rapid loss of cell viability in some conditions during the stationary phase compared to and . The present work aimed to evaluate the viability and cell cycle progression of representatives of species along their growth in an aerobic and discontinuous system. Cell growth, viability and DNA content were determined by turbidity, Trypan Blue staining, and flow cytometry, respectively. Results showed that (representing FEL group), and (SEL group) exhibited a typical G1/G0 (1C DNA) arrest during the stationary phase, as . Conversely, the three strains studied here of (SEL group) arrested at G2/M stages of cell cycle (2C DNA), and lost viability rapidly when enter the stationary phase. These results showed that have a unique cell cycle behavior that will contribute as a new eukaryotic model for future studies of genetic determinants of yeast cell cycle control and progression.
由于其对葡萄酒品质的贡献以及一些基因的丢失，主要与DNA修复和细胞周期过程相关，该属酵母在过去几年中声名狼藉。基于该属许多成员的基因组数据，它们被分为两个明确的进化枝：“快速进化谱系”（FEL）和“缓慢进化谱系”（SEL）。在此背景下，我们检测到与[具体菌株2]和[具体菌株3]相比，[具体菌株1]在稳定期的某些条件下细胞活力迅速丧失。本研究旨在评估该属物种代表在有氧和不连续系统中的生长过程中的活力和细胞周期进程。分别通过比浊法、台盼蓝染色和流式细胞术测定细胞生长、活力和DNA含量。结果表明，[具体菌株1]（代表FEL组）和[具体菌株2]（SEL组）在稳定期表现出典型的G1/G0（1C DNA）停滞，与[具体菌株3]相同。相反，这里研究的三株[具体菌株3]（SEL组）在细胞周期的G2/M阶段（2C DNA）停滞，进入稳定期后活力迅速丧失。这些结果表明，[具体菌株3]具有独特的细胞周期行为，这将为未来酵母细胞周期控制和进程的遗传决定因素研究提供一个新的真核模型。
