Biotechnology and Biomolecule Engineering Unit, National Center for Nuclear Energy, Science and Technology (CNESTEN), BP 1382, 10001, Rabat, Morocco.
Health and Environment Laboratory, Faculty of Sciences Aïn-Chock, Hassan II University, Km 8 Route d'El Jadida, Mâarif, BP 5366, 20100, Casablanca, Morocco.
Curr Microbiol. 2022 Aug 3;79(9):279. doi: 10.1007/s00284-022-02924-3.
The main purpose of this study was to investigate the protective effect of Rosmarinus officinalis, Origanum compactum, Lavandula angustifolia, and Eucalyptus globulus essential oils (EOs) against gamma-radiation-induced damages on Tetrahymena pyriformis growing in presence of cobalt-60 source. The chemical composition of the 4 EOs was analyzed by gas chromatography-mass spectrometry. The protective effects of EOs on growth, on morphology, and on some metabolic enzymes and antioxidant markers have been evaluated. Thus, addition of EOs significantly improves the growth parameters (generation number and time) in irradiating conditions. All EOs allowed restoring growth parameters over more than 90% compared to the controls. The morphological analysis indicated that T. pyriformis cells growing in irradiating conditions were able to regain their normal form in presence of the different EOs. Our results indicate that the 4 EOs also have protective effects on some metabolic enzymes. They allowed recovering totally or partially the glyceraldehyde 3-phosphate dehydrogenase and the succinate dehydrogenase activities compared to the controls. Moreover, the addition of EOs reduced the lipid peroxidation level and decreased the activities of catalase and superoxide dismutase induced by the gamma-radiation exposure. A more pronounced protective effect was found for O. compactum and L. angustifolia EOs compared to R. officinalis and E. globulus EOs. These results suggest that the studied EOs are efficient natural antioxidants that could offer protection against gamma-radiation-induced damages and can therefore be useful in clinical medicine.
本研究的主要目的是研究迷迭香、牛至、狭叶薰衣草和蓝桉精油（EOs）对在钴-60 源存在下生长的四膜虫的 γ 辐射损伤的保护作用。通过气相色谱-质谱法分析了 4 种 EO 的化学成分。评估了 EO 对生长、形态以及一些代谢酶和抗氧化标记物的保护作用。因此，添加 EO 可显著改善辐照条件下的生长参数（世代数和时间）。与对照组相比，所有 EO 都允许将生长参数恢复超过 90%。形态分析表明，在辐照条件下生长的四膜虫细胞能够在不同 EO 的存在下恢复其正常形态。我们的结果表明，这 4 种 EO 对一些代谢酶也具有保护作用。与对照组相比，它们使甘油醛-3-磷酸脱氢酶和琥珀酸脱氢酶的活性完全或部分恢复。此外，添加 EO 降低了由 γ 辐射暴露引起的脂质过氧化水平，并降低了过氧化氢酶和超氧化物歧化酶的活性。与迷迭香和蓝桉 EO 相比，牛至和狭叶薰衣草 EO 具有更显著的保护作用。这些结果表明，研究中的 EO 是有效的天然抗氧化剂，可提供对 γ 辐射损伤的保护作用，因此在临床医学中可能有用。
