Chemical Biology Unit, Institute of Nano Science and Technology (INST), Sector-81, Knowledge City, SAS Nagar, Punjab 140306, India.
Disease Biology Laboratory, Regional Centre for Biotechnology (RCB), National Capital Region Biotech Science Cluster, Faridabad, Haryana 121001, India.
Biomater Adv. 2022 May;136:212796. doi: 10.1016/j.bioadv.2022.212796. Epub 2022 Apr 11.
Inflammatory Bowel (IBD) is an umbrella term which includes Crohn's Disease (CD) and Ulcerative Colitis (UC). At present, therapies available for management of the UC includes, corticosteroid, immuno-suppressants and antibiotics are used for mild to moderate UC conditions which can cause nephrotoxicity, hepatotoxicity and cardiotoxicity. Hence, a novel therapeutic candidate having potent anti-inflammatory effect is urgently warranted for the management of UC. Melatonin has emerged as a potent anti-inflammatory agent. However, poor solubility limits its therapeutic potential. Therefore, colon targeted Eudragit-S-100 coated chitosan nanoparticles have been demonstrated to improve melatonin therapeutic efficacy. It was found that melatonin loaded chitosan and colon targeted chitosan nanoparticles had promising anti-inflammatory efficacy in terms of NO scavenging activity in an in-vitro LPS challenged macrophages. Also, colon targeted oral chitosan nano-formulation exhibited remarkable protection in an in vivo UC mice model by improving gross pathological parameters, histo-architectural protection, goblet cell depletion, and immune cells infiltration which can be extrapolated to clinical studies.
炎症性肠病（IBD）是一个总称，包括克罗恩病（CD）和溃疡性结肠炎（UC）。目前，用于 UC 管理的治疗方法包括皮质类固醇、免疫抑制剂和抗生素，用于轻度至中度 UC 病症，这些药物可能会引起肾毒性、肝毒性和心脏毒性。因此，迫切需要一种具有强大抗炎作用的新型治疗候选药物来治疗 UC。褪黑素已成为一种有效的抗炎剂。然而，较差的溶解性限制了其治疗潜力。因此，已经证明结肠靶向 Eudragit-S-100 包衣壳聚糖纳米粒可以提高褪黑素的治疗效果。研究发现，褪黑素负载的壳聚糖和结肠靶向壳聚糖纳米粒在体外 LPS 刺激的巨噬细胞中具有良好的 NO 清除活性，具有有前途的抗炎功效。此外，结肠靶向口服壳聚糖纳米制剂通过改善大体病理参数、组织形态保护、杯状细胞耗竭和免疫细胞浸润，在体内 UC 小鼠模型中表现出显著的保护作用，这可以推断到临床研究中。
