Department of Surgical Sciences, Faculty of Dentistry, Health Sciences Center, Kuwait University, Jabryia, Kuwait.
Kuwait Dental Administration, Kuwait Ministry of Health, Safat, Kuwait.
Clin Exp Dent Res. 2022 Dec;8(6):1331-1340. doi: 10.1002/cre2.644. Epub 2022 Aug 7.
Bone grafting is commonly used to reconstruct skeletal defects in the craniofacial region. Several bone augmentation models have been developed to evaluate bone formation using novel bone substitute materials. The aim of this study was to evaluate a surgical animal model for establishing a three-dimensional (3D) grafting environment in the animal's mandibular ramus for bone augmentation using the osseous shell technique, as in humans.
Osteological survey of New Zealand white (NZW) rabbit skull (Oryctolagus cuniculus): Initial osteological and imaging surveys were performed on a postmortem skull for a feasibility assessment of the surgical procedure. Postmortem pilot surgery and cone beam computed tomography imaging: a 3D osseous defect was created in the mandibular ramus through a submandibular incision. The osseous shell plates were stabilized with osteosynthesis fixation screws, and defects were filled with particular bone grafting material. In vivo surgical procedure: surgeries were conducted in four 8-week-old NZW rabbits utilizing two osseous shell materials: xenogeneic human cortical plates and autogenous rabbit cortical plates. The created 3D defects were filled using xenograft and allograft bone grafting materials. The healed defects were evaluated for bone formation after 12 weeks using histological and cone beam computed tomography imaging analysis.
Clinical analysis 12 weeks after surgery revealed the stability of the 3D grafted bone augmentation defects using the osseous shell technique. Imaging and histological analyses confirmed the effectiveness of this model in assessing bone formation.
The proposed animal model is a promising model with the potential to study various bone grafting materials for augmentation in the mandibular ramus using the osseous shell technique without compromising the health of the animal. The filled defects could be analyzed for osteogenesis, quantification of bone formation, and healing potential using histomorphometric analysis, in addition to 3D morphologic evaluation using radiation imaging.
在颅面区域，骨移植常用于重建骨骼缺损。已经开发了几种骨增强模型，以使用新型骨替代材料评估骨形成。本研究旨在评估一种手术动物模型，以在动物的下颌支中建立三维（3D）移植环境，用于骨增强，类似于人类的骨壳技术。
新西兰白兔（Oryctolagus cuniculus）颅骨的骨骼学调查：首先对死后颅骨进行骨骼学和影像学调查，以评估手术程序的可行性。死后初步手术和锥形束计算机断层扫描成像：通过下颌下切口在下颌支中创建 3D 骨缺损。使用骨合成固定螺钉稳定骨壳板，并使用特定的骨移植材料填充缺陷。体内手术程序：在四只 8 周龄的新西兰白兔中进行手术，使用两种骨壳材料：异种异体人皮质板和自体兔皮质板。使用同种异体和异体骨移植材料填充所创建的 3D 缺陷。在 12 周后，通过组织学和锥形束计算机断层扫描成像分析评估愈合缺陷中的骨形成。
手术后 12 周的临床分析显示，使用骨壳技术的 3D 移植骨增强缺陷具有稳定性。成像和组织学分析证实了该模型在评估骨形成方面的有效性。
所提出的动物模型是一种有前途的模型，具有使用骨壳技术在不影响动物健康的情况下在下颌支中研究各种骨移植材料增强的潜力。可以使用组织形态计量学分析除了放射成像的 3D 形态评估之外，对填充的缺陷进行成骨、骨形成的定量和愈合潜力的分析。
