Optimization for the production of a polyketone DMD from (Agaricomycetes) by submerged fermentation.

3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3,4)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus . However, the yield of DMD in solid culture of is very low and the production period are too long. In this work, efficient accumulation of 3,4DMD in by submerged fermentation is studied. The key fermentation factors of for DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance DMD production. A maximum DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO · 7HO, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The -DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of -DMD by submerged fermentation.