Division of Clinical Epidemiology and Aging Research, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
Medical Faculty Heidelberg, University of Heidelberg, Im Neuenheimer Feld 672, 69120 Heidelberg, Germany.
Cells. 2022 Aug 8;11(15):2462. doi: 10.3390/cells11152462.
We recently derived and validated a serum-based microRNA risk score (miR-score) which predicted colorectal cancer (CRC) occurrence with very high accuracy within 14 years of follow-up in a large population-based cohort. Here, we aimed to assess and compare the distribution of the miR-score among participants of screening colonoscopy at various stages of colorectal carcinogenesis. MicroRNAs (miRNAs) were profiled by quantitative-real-time-polymerase-chain-reaction in the serum samples of screening colonoscopy participants with CRC ( = 52), advanced colorectal adenoma (AA, = 100), non-advanced colorectal adenoma (NAA, = 88), and participants free of colorectal neoplasms ( = 173). The mean values of the miR-score were compared between groups by the Mann-Whitney U test. The associations of the miR-score with risk for colorectal neoplasms were evaluated using logistic regression analyses. MicroRNA risk scores were significantly higher among participants with AA than among those with NAA ( = 0.027) and those with CRC ( = 0.014), whereas no statistically significant difference was seen between those with NAA and those with no colorectal neoplasms ( = 0.127). When comparing adjacent groups, miR-scores were inversely associated with CRC versus AA and positively associated with AA versus NAA [odds ratio (OR), 0.37 (95% confidence interval (CI), 0.16-0.86) and OR, 2.22 (95% CI, 1.06-4.64) for the top versus bottom tertiles, respectively]. Our results are consistent with the hypothesis that a high miR-score may be indicative of an increased CRC risk by an increased tendency of progression from non-advanced to advanced colorectal neoplasms, along with a change of the miR-patterns after CRC manifestation.
我们最近提出并验证了一种基于血清的 microRNA 风险评分（miR-score），该评分在大规模基于人群的队列中，通过 14 年的随访，能够非常准确地预测结直肠癌（CRC）的发生。在这里，我们旨在评估和比较 miR-score 在不同结直肠癌变阶段的筛查结肠镜检查参与者中的分布情况。通过定量实时聚合酶链反应（PCR）对筛查结肠镜检查参与者的血清样本进行 microRNAs（miRNAs）分析，CRC（=52）、高级结直肠腺瘤（AA，=100）、非高级结直肠腺瘤（NAA，=88）和无结直肠肿瘤的参与者（=173）。采用 Mann-Whitney U 检验比较各组 miR-score 的平均值。采用 logistic 回归分析评估 miR-score 与结直肠肿瘤风险的关系。AA 组的 miR-score 明显高于 NAA 组（=0.027）和 CRC 组（=0.014），而 NAA 组与无结直肠肿瘤组之间的差异无统计学意义（=0.127）。在比较相邻组时，miR-scores 与 CRC 与 AA 相比呈负相关，与 AA 与 NAA 相比呈正相关[优势比（OR），0.37（95%置信区间（CI），0.16-0.86）和 OR，2.22（95% CI，1.06-4.64）分别为最高与最低三分位数]。我们的结果与以下假设一致：高 miR-score 可能表明从非高级到高级结直肠肿瘤的进展趋势增加，从而增加 CRC 风险，同时在 CRC 表现后发生 miR 模式的变化。
