Department of Chemistry, Seoul Women's University, 621 Hwarang-ro, Nowon-gu, Seoul 01797, Korea.
Molecules. 2022 Jul 27;27(15):4795. doi: 10.3390/molecules27154795.
Among various volatile organic compounds (VOCs) emitted from human skin, -2-nonenal, benzothiazole, hexyl salicylate, α-hexyl cinnamaldehyde, and isopropyl palmitate are key indicators associated with the degrees of aging. In our study, extraction and determination methods of human body odor are newly developed using headspace-in needle microextraction (HS-INME). The adsorbent was synthesized with graphene oxide:polyaniline/zinc nanorods/zeolitic imidazolate framework-8 (GO:PANI/ZNRs/ZIF-8). Then, a wire coated with the adsorbent was placed into the adsorption kit to be directly exposed to human skin as in vivo sampling and inserted into the needle so that it was able to be desorbed at the GC injector. The adsorption kit was made in-house with a 3D printer. For the in vitro method, the wire coated with the adsorbent was inserted into the needle and exposed to the headspace of the vial. When a cotton T-shirt containing body odor was transferred to a vial, the headspace of the vial was saturated with body odor VOCs. After volatile organic compounds were adsorbed in the dynamic mode, the needle was transferred to the injector for analysis of the volatile organic compounds by gas chromatography/mass spectrometry (GC/MS). The conditions of adsorbent fabrication and extraction for body odor compounds were optimized by response surface methodology (RSM). In conclusion, it was able to synthesize GO:PANI/ZNRs/ZIF-8 at the optimal condition and applicable to both in vivo and in vitro methods for body odor VOCs analysis.
在人体皮肤排放的各种挥发性有机化合物 (VOCs) 中， -2-壬烯醛、苯并噻唑、己基水杨酸酯、α-己基肉桂醛和棕榈酸异丙酯是与衰老程度相关的关键指标。在我们的研究中，使用顶空-针微萃取 (HS-INME) 新开发了人体气味的提取和测定方法。吸附剂是用氧化石墨烯：聚苯胺/锌纳米棒/沸石咪唑酯骨架-8 (GO:PANI/ZNRs/ZIF-8) 合成的。然后，将涂有吸附剂的金属丝放入吸附试剂盒中，直接暴露于人体皮肤进行体内采样，并插入针中，以便在 GC 进样器中进行解吸。吸附试剂盒是用 3D 打印机自制的。对于体外方法，将涂有吸附剂的金属丝插入针中，并暴露于小瓶的顶空部分。当含有体臭的棉质 T 恤转移到小瓶中时，小瓶的顶空部分就会充满体臭 VOCs。在动态模式下吸附挥发性有机化合物后，将针转移到进样器中，通过气相色谱/质谱 (GC/MS) 分析挥发性有机化合物。通过响应面法 (RSM) 优化了用于体臭化合物的吸附剂制备和提取条件。总之，能够在最佳条件下合成 GO:PANI/ZNRs/ZIF-8，并适用于体内和体外方法分析体臭 VOCs。
