Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Korea.
KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Korea.
Sensors (Basel). 2022 Aug 8;22(15):5929. doi: 10.3390/s22155929.
Non-specific binding (NSB) is one of the important issues in biosensing performance. Herein, we designed a strategy for removing non-specific binding including anti-mouse IgG antibody and bovine serum albumin (BSA) by utilizing anisotropic cadmium selenide tetrapods (CdSe TPs) in a vortex flow. The shear force on the tetrapod nanoparticles was increased by controlling the rotation rate of the vortex flow from 0 rpm to 1000 rpm. As a result, photoluminescence (PL) signals of fluorescein (FITC)-conjugated protein, anti-mouse IgG antibody-FITC and bovine serum albumin (BSA)-FITC, were reduced by 35% and 45%, respectively, indicating that NSB can be removed under vortex flow. In particular, simultaneous NSB removal and protein capture can be achieved even with mixture solutions of target antibodies and anti-mouse IgG antibodies by applying cyclic mode vortex flow on anisotropic CdSe TPs. These results demonstrate successfully that NSB can be diminished by rotating CdSe TPs to generate shear force under vortex flow. This study opens up new research protocols for utilization of anisotropic nanoparticles under vortex flow, which increases the feasibility of protein capture and non-specific proteins removal for biosensors.
非特异性结合（NSB）是生物传感性能中的一个重要问题。在此，我们设计了一种通过在涡流中利用各向异性硒化镉四足体（CdSe TPs）去除非特异性结合物（包括抗小鼠 IgG 抗体和牛血清白蛋白（BSA））的策略。通过将涡流的转速从 0 rpm 控制到 1000 rpm，增加了四足体纳米颗粒上的剪切力。结果，荧光素（FITC）缀合蛋白、抗小鼠 IgG 抗体-FITC 和牛血清白蛋白（BSA）-FITC 的光致发光（PL）信号分别降低了 35%和 45%，表明在涡流下可以去除 NSB。特别地，通过在各向异性 CdSe TPs 上施加循环模式涡流，即使是目标抗体和抗小鼠 IgG 抗体的混合溶液也可以实现同时去除 NSB 和捕获蛋白质。这些结果成功地证明了通过旋转 CdSe TPs 在涡流中产生剪切力可以减少 NSB。这项研究为利用涡流中的各向异性纳米颗粒开辟了新的研究方案，增加了生物传感器中蛋白质捕获和非特异性蛋白质去除的可行性。
