格列本脲可减轻脑出血后小胶质细胞激活引起的脑水肿。
Glibenclamide attenuates brain edema associated with microglia activation after intracerebral hemorrhage.
机构信息
Department of Neurological Surgery, Division of Neurosurgery, Nihon University School of Medicine, Tokyo, Japan.
Department of Neurological Surgery, Division of Neurosurgery, Nihon University School of Medicine, Tokyo, Japan.
出版信息
Neurochirurgie. 2022 Dec;68(6):589-594. doi: 10.1016/j.neuchi.2022.07.009. Epub 2022 Aug 10.
OBJECTIVE
Glibenclamide, Sulfonylurea receptor 1 antagonist, reduces brain edema after cerebral hemorrhage. However, the effects of glibenclamide on microglial activation and inflammatory cell infiltration after cerebral hemorrhage are unclear. The present study investigated the effect of glibenclamide on microglial activation and inflammatory cell infiltration in a rat cerebral hemorrhage model.
METHODS
A collagenase intracerebral injection model was used to cause cerebral hemorrhage in rats. After injury, glibenclamide was continuously administered at 1.0μL/h for 24hours. We evaluated hematoma volume, brain edema, expression of ABCC8, galectin-3 and CD11b, and anti-Iba-1 antibody staining.
RESULTS
Glibenclamide significantly reduced water content. Meanwhile, glibenclamide significantly reduced expression of galectin-3 and CD11b in the cerebral cortex and putamen on the bleeding side. Immunohistochemical staining confirmed that glibenclamide attenuated activation of microglia around the hematoma.
CONCLUSIONS
Glibenclamide reduced microglial activation and infiltration of inflammatory cells, resulting in amelioration of cerebral edema.
目的
格列本脲是磺酰脲受体 1 拮抗剂，可减轻脑出血后的脑水肿。然而，格列本脲对脑出血后小胶质细胞激活和炎症细胞浸润的影响尚不清楚。本研究旨在探讨格列本脲对脑出血大鼠模型中小胶质细胞激活和炎症细胞浸润的影响。
方法
采用胶原酶脑内注射法制作大鼠脑出血模型。损伤后，格列本脲以 1.0μL/h 的速度连续给药 24 小时。我们评估了血肿体积、脑水肿、ABCC8、半乳糖凝集素-3 和 CD11b 的表达以及抗-Iba-1 抗体染色。
结果
格列本脲显著降低了水含量。同时，格列本脲显著降低了出血侧皮质和壳核中 galectin-3 和 CD11b 的表达。免疫组织化学染色证实，格列本脲减弱了血肿周围小胶质细胞的激活。
结论
格列本脲减少了小胶质细胞的激活和炎症细胞的浸润，从而减轻了脑水肿。
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