流感病毒血凝素的抗原性糖多肽HA1和HA2。IV. 分离的血凝素糖多肽的免疫原性特性。

Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. IV. Immunogenic properties of separated haemagglutinin glycopolypeptides.

作者信息

Styk B, Russ G, Poláková K

出版信息

Acta Virol. 1979 Jan;23(1):9-20.

Abstract

Highly purified haemagglutinin glycopolypeptides HA1 and HA2 were effective in eliciting an antibody response. HA2 had a markedly greater immunogenic potential than HA1. In gel double immunodiffusion, sera from rabbits immunized with HA2 produced more distinct precipitin lines than sera obtained by immunization with HA1. Both kinds of rabbit sera gave precipitation with homologous antigen and with bromelain-released and purified haemagglutinin (B-HA). In radioimmunoassay, sera from rabbits immunized with HA2 revealed considerable titres for 125I-labelled HA2 binding and reacted preferentially with 125I-labelled HA2. In general, sera from rabbits immunized with HA1 exhibited low titres for 125I-labelled HA1 binding: usually they reacted also with 125I-labelled B-HA and 125I-labelled HA2. Only rabbits injected with a few doses of HA1 at short intervals revealed preferential binding for 125I-labelled HA1. Glycopolypeptides HA1 and HA2 failed to induce haemagglutination-inhibiting and virus neutralizing antibodies in rabbits.

摘要

高度纯化的血凝素糖多肽HA1和HA2能有效引发抗体反应。HA2的免疫原性潜力明显大于HA1。在凝胶双向免疫扩散试验中，用HA2免疫的兔血清产生的沉淀线比用HA1免疫获得的血清产生的沉淀线更清晰。两种兔血清都能与同源抗原以及菠萝蛋白酶释放并纯化的血凝素（B-HA）发生沉淀反应。在放射免疫测定中，用HA2免疫的兔血清对125I标记的HA2结合显示出相当高的滴度，并且优先与125I标记的HA2发生反应。一般来说，用HA1免疫的兔血清对125I标记的HA1结合显示出低滴度：通常它们也会与125I标记的B-HA和125I标记的HA2发生反应。只有短时间间隔注射几剂HA1的兔子对125I标记的HA1表现出优先结合。糖多肽HA1和HA2未能在兔子体内诱导血凝抑制抗体和病毒中和抗体。