Ajmera Transplant Program, University Health Network, Toronto General Hospital.
Ajmera Transplant Program, University Health Network, Toronto General Hospital; Department of General, Visceral, and Transplantation Surgery, University Hospital Essen.
J Vis Exp. 2022 Jul 27(185). doi: 10.3791/63905.
Pancreas transplantation (PTx) is a curative treatment for people who live with the burden of a diagnosis of diabetes mellitus (DM). However, due to organ shortages and increasing numbers of patients being listed for PTx, new strategies are needed to increase the number of available grafts for transplantation. Static cold storage (SCS) is considered the gold standard for standard criteria organs. However, standard criteria donors (SCD) are becoming scarce and new strategies that can increase the rate of organ acceptance from extended criteria donors (ECD) are urgently needed. Normothermic ex vivo perfusion (NEVP) is one of the strategies that has become increasingly popular over the past couple of decades. This preservation method has already been used successfully in other organs (liver, kidneys, and lungs) but has been minimally explored in pancreas transplantation. The few papers that describe the method for pancreas show little success, edema being one of the major issues. The following manuscript describes the successful NEVP method and setup developed by our group to perfuse swine pancreas.
胰腺移植 (PTx) 是治疗患有糖尿病 (DM) 人群的一种有治愈可能的治疗方法。然而，由于器官短缺和越来越多的患者被列入 PTx 名单，需要新的策略来增加可用于移植的供体数量。静态冷保存 (SCS) 被认为是标准标准器官的金标准。然而，标准标准供体 (SCD) 越来越稀缺，迫切需要能够提高扩展标准供体 (ECD) 器官接受率的新策略。常温离体灌流 (NEVP) 是过去几十年来越来越受欢迎的策略之一。这种保存方法已成功应用于其他器官（肝脏、肾脏和肺部），但在胰腺移植中很少被探索。描述该方法的少数几篇论文显示效果甚微，水肿是主要问题之一。以下手稿描述了我们小组开发的成功的 NEVP 方法和设置，用于灌注猪胰腺。
