Su Kunyang, Li Xue, Lu Tianxiang, Mou Yiwen, Liu Na, Song Mingming, Yu Ze
School of Environmental Science & Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, PR China.
School of Environmental Science & Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, PR China.
Chemosphere. 2022 Nov;307(Pt 3):136047. doi: 10.1016/j.chemosphere.2022.136047. Epub 2022 Aug 14.
For the sustainable development of the environment, to reduce the high cost and low productivity of microalgae biofuel, nine microalgae strains were screened to study the growh and nutrient removal properties under heterotrophic culture by using the waste carbon source of volatile fatty acids (VFAs). Chlorella sorokiniana (C.sorokiniana) was selected as the best strain with the highest biomass concentration of 0.77 g L, specific growth rate of 0.25 d, biomass productivity of 91.43 mg L d, total nitrogen removal efficiency of 95.96% and total phosphorus removal efficiency of 93.42%. To study the utilization potential of acid-producing wastewater by heterotrophic microalgae, actual acid-producing wastewater was recycled three times for the utilization of C.sorokiniana. After the three utilization cultivation, the removal rates of COD, total nitrogen, ammonia nitrogen, and total phosphorus were 74.44%, 88.05%, 79.08%, and 82.69%, respectively. The total utilization rates of acetic acid, propionic acid, and butyric acid were 58.99%, 70.54%, and 81.52%, respectively. In addition, the highest lipid content of 39.15% and protein content of 42.43% achieved at the third cultivation. After the first cultivation, the composition and diversity of the microbial community structure changed dramatically, with Protebacteria, Bacteroidota, Hydrogenophaga, and Algoriphagus becoming enriched. These results showed a promising way of coupling wastewater treatment with biomass production for long-term sustainability of microalgae lipid production.
为实现环境的可持续发展，降低微藻生物燃料成本高、生产率低的问题，筛选了9株微藻菌株，利用挥发性脂肪酸（VFAs）废碳源研究其在异养培养条件下的生长及营养物去除特性。其中，索氏小球藻（C. sorokiniana）被选为最佳菌株，其生物量浓度最高可达0.77 g/L，比生长速率为0.25 d⁻¹，生物量生产率为91.43 mg/(L·d)，总氮去除效率为95.96%，总磷去除效率为93.42%。为研究异养微藻对产酸废水的利用潜力，将实际产酸废水循环利用3次用于索氏小球藻培养。经过3次利用培养后，化学需氧量（COD）、总氮、氨氮和总磷的去除率分别为74.44%、88.05%、79.08%和82.69%。乙酸、丙酸和丁酸的总利用率分别为58.99%、70.54%和81.52%。此外，第三次培养时脂质含量最高达到39.15%，蛋白质含量达到42.43%。第一次培养后微生物群落结构的组成和多样性发生了显著变化，变形菌门、拟杆菌门、嗜氢菌属和噬藻体属变得丰富。这些结果为微藻脂质生产的长期可持续性提供了一种将废水处理与生物质生产相结合的可行方法。
