• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种新型长非编码 RNA SP100-AS1 通过海绵吸附 miR-622 和稳定 ATG3 诱导结直肠癌的放射抵抗。

A novel long noncoding RNA SP100-AS1 induces radioresistance of colorectal cancer via sponging miR-622 and stabilizing ATG3.

机构信息

Tumor Biological Diagnosis and Treatment Center, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.

Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, 213003, China.

出版信息

Cell Death Differ. 2023 Jan;30(1):111-124. doi: 10.1038/s41418-022-01049-1. Epub 2022 Aug 17.

DOI:10.1038/s41418-022-01049-1
PMID:35978049
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9883267/
Abstract

Although radiotherapy is an essential modality in the treatment of colorectal cancer (CRC), the incidence of radioresistance remains high clinically. Long noncoding RNAs (lncRNAs) reportedly play critical roles in CRC radioresistance by regulating genes or proteins at the transcriptional or post-translational levels. This study aimed to identify novel lncRNAs involved in radioresistance. We found that SP100-AS1 (lncRNA targeting antisense sequence of SP100 gene) was upregulated in radioresistant CRC patient tissues using RNA-seq analysis. Importantly, knockdown of SP100-AS1 significantly reduced radioresistance, cell proliferation, and tumor formation in vitro and in vivo. Mechanistically, mass spectrometry and bioinformatics analyses were used to identify the interacting proteins and microRNAs of SP100-AS1, respectively. Moreover, SP100-AS1 was found to interact with and stabilize ATG3 protein through the ubiquitination-dependent proteasome pathway. In addition, it could serve as a sponge for miR-622, which targeted ATG3 mRNA and affected autophagic activity. Thus, lncRNA SP100-AS1 could act as a radioresistance factor in CRC patients via RNA sponging and protein stabilizing mechanisms. In conclusion, the present study indicates that SP100-AS1/miR-622/ATG3 axis contributes to radioresistance and autophagic activity in CRC patients, suggesting it has huge prospects as a therapeutic target for improving CRC response to radiation therapy.

摘要

虽然放射治疗是治疗结直肠癌（CRC）的重要手段，但临床上仍然存在放射抵抗的发生率较高的问题。长链非编码 RNA（lncRNA）据报道通过在转录或翻译后水平调节基因或蛋白质在 CRC 放射抵抗中发挥关键作用。本研究旨在鉴定参与放射抵抗的新的 lncRNA。我们通过 RNA-seq 分析发现，SP100-AS1（靶向 SP100 基因反义序列的 lncRNA）在放射抵抗的 CRC 患者组织中上调。重要的是，SP100-AS1 的敲低显著降低了体外和体内的放射抵抗、细胞增殖和肿瘤形成。机制上，分别使用质谱分析和生物信息学分析来鉴定 SP100-AS1 的相互作用蛋白和 microRNA。此外，发现 SP100-AS1 通过泛素化依赖的蛋白酶体途径与 ATG3 蛋白相互作用并稳定其蛋白水平。此外，它可以作为 miR-622 的海绵，miR-622 靶向 ATG3 mRNA 并影响自噬活性。因此，lncRNA SP100-AS1 可通过 RNA 海绵和蛋白稳定机制在 CRC 患者中作为放射抵抗因子发挥作用。总之，本研究表明 SP100-AS1/miR-622/ATG3 轴参与 CRC 患者的放射抵抗和自噬活性，表明其作为提高 CRC 对放射治疗反应的治疗靶点具有巨大的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/3edd76389abb/41418_2022_1049_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/a57f1aa02aaf/41418_2022_1049_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/dabaf84d5efb/41418_2022_1049_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/30263a527016/41418_2022_1049_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/4d64365a7807/41418_2022_1049_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/09867e2aba47/41418_2022_1049_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/0a740c57a45e/41418_2022_1049_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/625ddaba9ebb/41418_2022_1049_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/3edd76389abb/41418_2022_1049_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/a57f1aa02aaf/41418_2022_1049_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/dabaf84d5efb/41418_2022_1049_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/30263a527016/41418_2022_1049_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/4d64365a7807/41418_2022_1049_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/09867e2aba47/41418_2022_1049_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/0a740c57a45e/41418_2022_1049_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/625ddaba9ebb/41418_2022_1049_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9f1/9883267/3edd76389abb/41418_2022_1049_Fig8_HTML.jpg

相似文献

1
A novel long noncoding RNA SP100-AS1 induces radioresistance of colorectal cancer via sponging miR-622 and stabilizing ATG3.一种新型长非编码 RNA SP100-AS1 通过海绵吸附 miR-622 和稳定 ATG3 诱导结直肠癌的放射抵抗。
Cell Death Differ. 2023 Jan;30(1):111-124. doi: 10.1038/s41418-022-01049-1. Epub 2022 Aug 17.
2
LncRNA OTUD6B-AS1 overexpression promoted GPX4-mediated ferroptosis to suppress radioresistance in colorectal cancer.LncRNA OTUD6B-AS1 过表达促进 GPX4 介导的铁死亡以抑制结直肠癌细胞的放射抵抗。
Clin Transl Oncol. 2023 Nov;25(11):3217-3229. doi: 10.1007/s12094-023-03193-7. Epub 2023 May 15.
3
Long noncoding RNA FGD5-AS1 promotes colorectal cancer cell proliferation, migration, and invasion through upregulating CDCA7 via sponging miR-302e.长链非编码 RNA FGD5-AS1 通过海绵吸附 miR-302e 上调 CDCA7 促进结直肠癌细胞增殖、迁移和侵袭。
In Vitro Cell Dev Biol Anim. 2019 Sep;55(8):577-585. doi: 10.1007/s11626-019-00376-x. Epub 2019 Jul 22.
4
Upregulated lncRNA Cyclin-dependent kinase inhibitor 2B antisense RNA 1 induces the proliferation and migration of colorectal cancer by miR-378b/CAPRIN2 axis.上调的 lncRNA 细胞周期蛋白依赖性激酶抑制剂 2B 反义 RNA 1 通过 miR-378b/CAPRIN2 轴诱导结直肠癌细胞的增殖和迁移。
Bioengineered. 2021 Dec;12(1):5476-5490. doi: 10.1080/21655979.2021.1961656.
5
LncRNA TTN-AS1 sponges miR-376a-3p to promote colorectal cancer progression via upregulating KLF15.长链非编码 RNA TTN-AS1 通过海绵吸附 miR-376a-3p 促进结直肠癌细胞的进展，上调 KLF15。
Life Sci. 2020 Mar 1;244:116936. doi: 10.1016/j.lfs.2019.116936. Epub 2019 Oct 11.
6
Non-coding RNA MFI2-AS1 promotes colorectal cancer cell proliferation, migration and invasion through miR-574-5p/MYCBP axis.非编码 RNA MFI2-AS1 通过 miR-574-5p/MYCBP 轴促进结直肠癌细胞增殖、迁移和侵袭。
Cell Prolif. 2019 Jul;52(4):e12632. doi: 10.1111/cpr.12632. Epub 2019 May 16.
7
LncRNA DICER1-AS1 promotes colorectal cancer progression by activating the MAPK/ERK signaling pathway through sponging miR-650.LncRNA DICER1-AS1 通过海绵吸附 miR-650 激活 MAPK/ERK 信号通路促进结直肠癌进展。
Cancer Med. 2023 Apr;12(7):8351-8366. doi: 10.1002/cam4.5550. Epub 2023 Jan 27.
8
Long non-coding RNA TP73-AS1 sponges miR-194 to promote colorectal cancer cell proliferation, migration and invasion via up-regulating TGFα.长链非编码 RNA TP73-AS1 通过海绵吸附 miR-194 促进结直肠癌细胞增殖、迁移和侵袭并上调 TGFα。
Cancer Biomark. 2018;23(1):145-156. doi: 10.3233/CBM-181503.
9
SP1-induced PROX1-AS1 contributes to tumor progression by regulating miR-326/FBXL20 axis in colorectal cancer.SP1 诱导的 PROX1-AS1 通过调节 miR-326/FBXL20 轴促进结直肠癌的肿瘤进展。
Cell Signal. 2023 Jan;101:110503. doi: 10.1016/j.cellsig.2022.110503. Epub 2022 Oct 29.
10
H3K27 acetylation-induced lncRNA EIF3J-AS1 improved proliferation and impeded apoptosis of colorectal cancer through miR-3163/YAP1 axis.H3K27 乙酰化诱导的长链非编码 RNA EIF3J-AS1 通过 miR-3163/YAP1 轴促进结直肠癌的增殖并抑制其凋亡。
J Cell Biochem. 2020 Feb;121(2):1923-1933. doi: 10.1002/jcb.29427. Epub 2019 Nov 11.

引用本文的文献

1
Role of autophagy‑modulating long non‑coding RNAs in tumor radioresistance (Review).自噬调节性长链非编码RNA在肿瘤放射抗性中的作用（综述）
Oncol Rep. 2025 Nov;54(5). doi: 10.3892/or.2025.8975. Epub 2025 Aug 24.
2
Duodenal Adenocarcinoma Is Characterized by Acidity, High Infiltration of Macrophage, and Activated Linc01559-GRSF1 Axis.十二指肠腺癌的特征为酸性、巨噬细胞高度浸润以及Linc01559-GRSF1轴激活。
Biomedicines. 2025 Jun 30;13(7):1611. doi: 10.3390/biomedicines13071611.
3
The lncRNA MAGI2-AS3 in peripheral blood mononuclear cells: a valuable biomarker for diagnosis and prognosis prediction of breast cancer.

本文引用的文献

1
Role of long non-coding RNA H19 in therapy resistance of digestive system cancers.长链非编码 RNA H19 在消化系统癌症治疗抵抗中的作用。
Mol Med. 2021 Jan 5;27(1):1. doi: 10.1186/s10020-020-00255-2.
2
Gene regulation by long non-coding RNAs and its biological functions.长非编码 RNA 的基因调控及其生物学功能。
Nat Rev Mol Cell Biol. 2021 Feb;22(2):96-118. doi: 10.1038/s41580-020-00315-9. Epub 2020 Dec 22.
3
Inhibiting autophagy to prevent drug resistance and improve anti-tumor therapy.抑制自噬以预防耐药性并改善抗肿瘤治疗。
外周血单个核细胞中的长链非编码RNA MAGI2-AS3：一种用于乳腺癌诊断和预后预测的有价值生物标志物。
PeerJ. 2025 Jun 30;13:e19694. doi: 10.7717/peerj.19694. eCollection 2025.
4
Targeting epigenetic regulators as a promising avenue to overcome cancer therapy resistance.将表观遗传调节因子作为克服癌症治疗耐药性的一条有前景的途径。
Signal Transduct Target Ther. 2025 Jul 18;10(1):219. doi: 10.1038/s41392-025-02266-z.
5
Small nuclear ribonucleoprotein polypeptide B2 regulated by SNHG4/miR-204-5p axis inhibits ferroptosis to aggravate the progression of hepatocellular carcinoma.由SNHG4/miR-204-5p轴调控的小核核糖核蛋白多肽B2抑制铁死亡，从而加剧肝细胞癌的进展。
Discov Oncol. 2025 Jul 16;16(1):1349. doi: 10.1007/s12672-025-03192-w.
6
Long Non-Coding RNA LOC401312 Induces Radiosensitivity Through Upregulation of CPS1 in Non-Small Cell Lung Cancer.长链非编码RNA LOC401312通过上调CPS1诱导非小细胞肺癌的放射敏感性
Int J Mol Sci. 2025 Jun 19;26(12):5865. doi: 10.3390/ijms26125865.
7
LncRNA-Protein Interactions: A Key to Deciphering LncRNA Mechanisms.长链非编码RNA-蛋白质相互作用：破解长链非编码RNA机制的关键
Biomolecules. 2025 Jun 17;15(6):881. doi: 10.3390/biom15060881.
8
The multifaceted role of microRNAs in colorectal cancer: pathogenesis and therapeutic implications.微小RNA在结直肠癌中的多方面作用：发病机制及治疗意义
Noncoding RNA Res. 2025 May 23;14:65-95. doi: 10.1016/j.ncrna.2025.05.012. eCollection 2025 Oct.
9
The highly expressed in colorectal cancer cells activates smoothened to drive glycolysis and promote cancer cell growth and radiotherapy resistance.在结肠直肠癌细胞中高表达的（蛋白）激活了 smoothened 以驱动糖酵解并促进癌细胞生长和放疗抗性。 （注：原文句子不完整，缺少主语等关键信息，翻译只能根据现有内容尽量完善语义）
J Gastrointest Oncol. 2025 Apr 30;16(2):415-434. doi: 10.21037/jgo-2025-193. Epub 2025 Apr 27.
10
LncRNA FTX promotes colorectal cancer radioresistance through disturbing redox balance and inhibiting ferroptosis miR-625-5p/SCL7A11 axis.长链非编码RNA FTX通过扰乱氧化还原平衡和抑制铁死亡的miR-625-5p/SCL7A11轴促进结直肠癌放疗抵抗。
World J Gastroenterol. 2025 Apr 28;31(16):104305. doi: 10.3748/wjg.v31.i16.104305.
Life Sci. 2021 Jan 15;265:118745. doi: 10.1016/j.lfs.2020.118745. Epub 2020 Nov 10.
4
Immunotherapy efficacy on mismatch repair-deficient colorectal cancer: From bench to bedside.免疫疗法对错配修复缺陷型结直肠癌的疗效：从基础到临床。
Biochim Biophys Acta Rev Cancer. 2020 Dec;1874(2):188447. doi: 10.1016/j.bbcan.2020.188447. Epub 2020 Oct 6.
5
Nanoparticles (NPs)-Meditated LncRNA AFAP1-AS1 Silencing to Block Wnt/-Catenin Signaling Pathway for Synergistic Reversal of Radioresistance and Effective Cancer Radiotherapy.纳米颗粒介导的长链非编码RNA AFAP1-AS1沉默以阻断Wnt/β-连环蛋白信号通路，实现协同逆转放射抗性及有效的癌症放射治疗
Adv Sci (Weinh). 2020 Aug 5;7(18):2000915. doi: 10.1002/advs.202000915. eCollection 2020 Sep.
6
Mitochondrial DNA drives abscopal responses to radiation that are inhibited by autophagy.线粒体 DNA 驱动放疗的远隔效应，自噬会抑制这种远隔效应。
Nat Immunol. 2020 Oct;21(10):1160-1171. doi: 10.1038/s41590-020-0751-0. Epub 2020 Aug 3.
7
Hydroxychloroquine-loaded hollow mesoporous silica nanoparticles for enhanced autophagy inhibition and radiation therapy.载羟氯喹的中空介孔硅纳米粒增强自噬抑制和放射治疗。
J Control Release. 2020 Sep 10;325:100-110. doi: 10.1016/j.jconrel.2020.06.025. Epub 2020 Jul 1.
8
Aberrant expression of lncRNA MALAT1 modulates radioresistance in colorectal cancer in vitro via miR-101-3p sponging.长链非编码 RNA MALAT1 的异常表达通过海绵吸附 miR-101-3p 调节结直肠癌的体外放射抵抗。
Exp Mol Pathol. 2020 Aug;115:104448. doi: 10.1016/j.yexmp.2020.104448. Epub 2020 May 5.
9
The role of long noncoding RNAs in hepatocellular carcinoma.长链非编码 RNA 在肝细胞癌中的作用。
Mol Cancer. 2020 Apr 15;19(1):77. doi: 10.1186/s12943-020-01188-4.
10
Role of non-coding RNAs and RNA modifiers in cancer therapy resistance.非编码 RNA 和 RNA 修饰物在癌症治疗耐药中的作用。
Mol Cancer. 2020 Mar 2;19(1):47. doi: 10.1186/s12943-020-01171-z.