El-Hanboushy Sara, Marzouk Hoda M, Fayez Yasmin M, Abdelkawy Mohamed, Lotfy Hayam M
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Future University in Egypt, 11835, Cairo, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562, Cairo, Egypt.
Sustain Chem Pharm. 2022 Oct;29:100806. doi: 10.1016/j.scp.2022.100806. Epub 2022 Aug 15.
Recent studies have reported that using certain antihypertensive therapies such as angiotensin II receptor blockers (ARBs) is associated with mitigation of fatal outcomes and enhancing clinical features of patients having hypertension during coronavirus pandemic. Thus, in the current work an innovative, effective, white and sustainable spectrophotometric technique called paired wavelength data processing technique (PWDPT) was developed for evaluation of recommended antihypertensive combination therapies incorporating candesartan cilexetil (CAN) and hydrochlorothiazide (HCT). This technique included three methods, namely, absorbance resolution (AR), amplitude resolution (PR) and ratio extraction (RE). Linearity ranges were (5.0 μg/mL - 50.0 μg/mL) and (2.0 μg/mL - 24.0 μg/mL) for CAN and HCT, respectively. Validation and confirmation of all suggested methods were conducted in accordance with ICH guidelines, producing satisfactory results within the accepted limits. Statistical comparison was achieved between the attained results from suggested methods and those attained from official methods, in which insignificant difference was existed. The suggested methods were successfully employed for identification of the studied drugs as well as determination of their spectral recognition and evaluation of the purity in their combined formulations. The proposed methods followed the principles of green analytical chemistry, where their greenness was evaluated and compared with the official potentiometric and HPLC methods via using four tools, namely, National Environmental Methods Index (NEMI), the Analytical Eco-Scale, the Green Analytical Procedure Index (GAPI) and Analytical greenness metric (AGREE) which affirmed the eco-friendly nature of the proposed methods. Moreover, studying the whiteness features was performed using the recently introduced RGB12 model. The acceptable results along with the sustainability, simplicity, affordability and low-cost of the proposed methods encourages their utilization in the quality control laboratories.
最近的研究报告称,在冠状病毒大流行期间,使用某些抗高血压疗法,如血管紧张素II受体阻滞剂(ARBs),与减轻致命后果以及改善高血压患者的临床特征有关。因此,在当前的工作中,开发了一种创新、有效、白色且可持续的分光光度技术,称为配对波长数据处理技术(PWDPT),用于评估包含坎地沙坦酯(CAN)和氢氯噻嗪(HCT)的推荐抗高血压联合疗法。该技术包括三种方法,即吸光度分辨率(AR)、幅度分辨率(PR)和比率提取(RE)。CAN和HCT的线性范围分别为(5.0μg/mL - 50.0μg/mL)和(2.0μg/mL - 24.0μg/mL)。所有建议方法的验证和确认均按照国际协调会议(ICH)指南进行,在可接受的限度内产生了令人满意的结果。对建议方法获得的结果与官方方法获得的结果进行了统计比较,其中不存在显著差异。建议的方法成功地用于鉴定所研究的药物,以及确定它们的光谱识别和评估其复方制剂的纯度。所提出的方法遵循绿色分析化学的原则,通过使用四种工具,即国家环境方法指数(NEMI)、分析生态尺度、绿色分析程序指数(GAPI)和分析绿色度指标(AGREE),对其绿色度进行了评估,并与官方电位滴定法和高效液相色谱法进行了比较,这些工具证实了所提出方法的生态友好性质。此外,使用最近引入的RGB12模型对白色特征进行了研究。所提出方法的可接受结果以及可持续性、简单性、可承受性和低成本鼓励它们在质量控制实验室中得到应用。