Kuspinar Goktan, Cakır Cihan, Kasapoglu Isıl, Saribal Seda, Oral Barbaros, Budak Ferah, Uncu Gurkan, Avcı Berrin
Department of Histology and Embryology, Bursa Uludag University School of Medicine, Bursa, Turkey.
Department of Gynecology and Obstetric ART Center, Bursa Uludag University School of Medicine, Bursa, Turkey.
J Obstet Gynaecol. 2022 Oct;42(7):3241-3247. doi: 10.1080/01443615.2022.2112018. Epub 2022 Aug 22.
The aim of this study was to determine whether Kisspeptin and Kisspeptin receptor in the follicular microenvironment is necessary for human oocyte maturation and fertilisation. The cumulus cell (CC) and follicle fluids (FF) obtained from the first aspirated follicles ( = 52) from 32 patients were divided into three groups considering nuclear maturation and fertilisation results of oocytes: (1) Metaphase I or germinal vesicle stage oocytes (incomplete nuclear maturation, = 10), (2) unfertilised metaphase II oocytes (incomplete cytoplasmic maturation, = 16), and (3) fertilised metaphase II oocytes (completed nuclear-cytoplasmic maturation, = 26). The gene expression levels were assessed by RT-PCR. The levels of Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) were measured by ELISA. There were no significant efficacy and gene expressions in cumulus cells in terms of oocyte nuclear maturation stage (Group 1, vs Group 2 + Group 3) (respectively = .49; = .45). In terms of the cytoplasmic maturation stage (Group 2, vs Group 3); and expressions in CCs were comparable (respectively = .07; = .08). In FFs, and concentrations were similar between all groups (respectively = .86; = .26). In conclusion, the relative and expressions in CC and also KISS1 and KISS1R level of FF were independent of oocytes nuclear and/or cytoplasmic maturation. Impact statement It has been demonstrated that Kisspeptin is an essential regulator of reproductive function and plays a key role in the modulation of GnRH secretion and gonadotropin release. Still, no information is available about the link between gene expression or concentration in the follicular microenvironment and oocyte development. The study has shown that the relative Kisspeptin ( and Kisspeptin receptor and expressions in cumulus cell (CC) and also KISS1 and KISS1R levels of follicle fluids (FF) were independent of oocytes nuclear and/or cytoplasmic maturation. Based on the findings, it is difficult to establish a concept that kisspeptin can directly induce oocyte maturation. Nevertheless, to confirm these findings, further studies with a larger sample size are needed.
本研究的目的是确定卵泡微环境中的亲吻素和亲吻素受体对于人类卵母细胞成熟和受精是否必要。根据卵母细胞的核成熟和受精结果，将从32例患者首次抽吸的卵泡（n = 52）中获得的卵丘细胞（CC）和卵泡液（FF）分为三组：（1）中期I或生发泡期卵母细胞（核成熟不完全，n = 10），（2）未受精的中期II卵母细胞（胞质成熟不完全，n = 16），以及（3）受精的中期II卵母细胞（核-胞质成熟完成，n = 26）。通过逆转录聚合酶链反应（RT-PCR）评估基因表达水平。通过酶联免疫吸附测定（ELISA）测量亲吻素（KISS1）和亲吻素受体（KISS1R）的水平。就卵母细胞核成熟阶段而言（第1组与第2组+第3组相比），卵丘细胞中无显著疗效和基因表达（分别为P = 0.49；P = 0.45）。就胞质成熟阶段而言（第2组与第3组相比），CCs中的KISS1和KISS1R表达具有可比性（分别为P = 0.07；P = 0.08）。在FFs中，所有组之间的KISS1和KISS1R浓度相似（分别为P = 0.86；P = 0.26）。总之，CC中KISS1和KISS1R的相对表达以及FF中的KISS1和KISS1R水平与卵母细胞的核和/或胞质成熟无关。影响声明 已证明亲吻素是生殖功能的重要调节因子，在促性腺激素释放激素（GnRH）分泌和促性腺激素释放的调节中起关键作用。然而，关于卵泡微环境中的基因表达或浓度与卵母细胞发育之间的联系尚无信息。该研究表明，卵丘细胞（CC）中亲吻素（KISS1）和亲吻素受体（KISS1R）的相对表达以及卵泡液（FF）中的KISS1和KISS1R水平与卵母细胞的核和/或胞质成熟无关。基于这些发现，很难确立亲吻素可直接诱导卵母细胞成熟的概念。然而，为证实这些发现，需要进行更大样本量的进一步研究。
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