流式细胞术诊断的犬淋巴结小 B 细胞淋巴瘤的临床结果和 Ki67 评估。

Clinical outcome and Ki67 evaluation in dogs with nodal small cell B-cell lymphoma diagnosed by flow cytometry.

机构信息

Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.

Flint Animal Cancer Center, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.

出版信息

J Vet Intern Med. 2022 Sep;36(5):1770-1781. doi: 10.1111/jvim.16515. Epub 2022 Aug 23.

DOI:10.1111/jvim.16515

PMID:35996942

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9511060/

Abstract

BACKGROUND

Nodal small cell B-cell lymphoma subtypes in dogs cannot be distinguished by flow cytometry and information regarding treatment, prognosis, and outcome are limited.

HYPOTHESIS/OBJECTIVES: Objectives were to describe outcome in dogs with nodal small cell B-cell lymphoma diagnosed by flow cytometry and correlate clinical and laboratory data with survival. We hypothesized that B-cell Ki67 expression measured by flow cytometry is associated with shorter progression free survival (PFS) and overall survival (OS).

ANIMALS

Forty-nine dogs with nodal small cell B-cell lymphoma, defined by >80% CD21+ B-cells by flow cytometry and small-sized B-cells by forward scatter.

METHODS

Retrospective study reviewing treatment and outcome data extracted from medical records. Percentage of Ki67-expressing B-cells was measured by flow cytometry. Clinical, laboratory, and flow cytometry data were assessed for association with outcome.

RESULTS

Median percentage of B-cell Ki67 was 41% (range, 3%-97%). Median PFS was 119 days and median OS was 222 days (n = 49). Among cases treated with CHOP-based chemotherapy (n = 32), median PFS was 70 days, median OS was 267 days, and 50% of cases achieved complete response. Low percentage of B-cell Ki67 (≤11%) was associated with prolonged OS by univariable analysis. Greater age, substage B, high B-cell CD25 expression and low B-cell CD21 and class II major histocompatibility complex expression by flow cytometry were independently associated with shorter OS.

CONCLUSIONS AND CLINICAL IMPORTANCE

Most nodal small cell B-cell lymphoma cases had aggressive disease. Low Ki67 expression can help identify cases with better prognosis. Age, substage, and flow cytometry variables are useful prognostic factors.

摘要

背景

犬类淋巴结小 B 细胞淋巴瘤亚型无法通过流式细胞术区分，且有关治疗、预后和结果的信息有限。

假设/目的：本研究旨在描述通过流式细胞术诊断为淋巴结小 B 细胞淋巴瘤的犬的预后，并将临床和实验室数据与生存情况相关联。我们假设流式细胞术测量的 B 细胞 Ki67 表达与无进展生存期（PFS）和总生存期（OS）较短相关。

动物

49 例诊断为淋巴结小 B 细胞淋巴瘤的犬，通过流式细胞术定义为>80%的 CD21+B 细胞和前向散射的小 B 细胞。

方法

回顾性研究，从病历中提取治疗和预后数据。通过流式细胞术测量 Ki67 表达的 B 细胞百分比。评估临床、实验室和流式细胞术数据与预后的相关性。

结果

中位 B 细胞 Ki67 百分比为 41%（范围，3%-97%）。中位 PFS 为 119 天，中位 OS 为 222 天（n=49）。在接受 CHOP 为基础的化疗治疗的病例中（n=32），中位 PFS 为 70 天，中位 OS 为 267 天，50%的病例达到完全缓解。单变量分析显示，低 B 细胞 Ki67 百分比（≤11%）与 OS 延长相关。年龄较大、亚分期 B、高 B 细胞 CD25 表达以及低 B 细胞 CD21 和 II 类主要组织相容性复合物表达与 OS 较短独立相关。