State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital & School of Stomatology, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China.
Department of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, 200433, People's Republic of China.
J Mater Chem B. 2022 Aug 31;10(34):6546-6556. doi: 10.1039/d2tb00853j.
Porous scaffolds hold promise in the treatment of bone defects for bone tissue engineering due to their interconnected porous structure and suitable mechanical properties. Herein, LAPONITE® (LAP), which is able to promote osteogenic differentiation, was introduced into regenerated silk fibroin (RSF) porous scaffolds. Due to hydrogen bonding and electrostatic interactions between RSF and LAP, RSF/LAP 3D porous scaffolds were successfully prepared. The pore size, porosity, and mechanical properties of the RSF/LAP 3D porous scaffolds were modulated during the preparation process. Evaluation of the proliferation of bone marrow mesenchymal stem cells (BMSCs) on the RSF/LAP 3D porous scaffolds indicated that the addition of LAP improved the adhesion and proliferation of cells. Additionally, alkaline phosphatase activity and osteospecific gene expression analysis showed that the RSF/LAP 3D porous scaffolds enhanced the osteogenic differentiation of BMSCs compared to the pristine RSF porous scaffolds, especially with a higher LAP content. The subcutaneous implantation of the RSF/LAP 3D porous scaffolds in rats demonstrated good histocompatibility . Therefore, RSF/LAP 3D porous scaffolds with good biocompatibility and biodegradability have good application prospects in the field of bone tissue engineering.
多孔支架由于其相互连通的多孔结构和合适的机械性能，在骨组织工程治疗骨缺损方面具有广阔的应用前景。本文将能够促进成骨分化的 LAPONITE®（LAP）引入再生丝素蛋白（RSF）多孔支架中。由于 RSF 和 LAP 之间存在氢键和静电相互作用，成功制备了 RSF/LAP 3D 多孔支架。在制备过程中，调节了 RSF/LAP 3D 多孔支架的孔径、孔隙率和力学性能。对骨髓间充质干细胞（BMSCs）在 RSF/LAP 3D 多孔支架上增殖的评价表明，添加 LAP 可以提高细胞的黏附和增殖。此外，碱性磷酸酶活性和骨特异性基因表达分析表明，与原始 RSF 多孔支架相比，RSF/LAP 3D 多孔支架增强了 BMSCs 的成骨分化，尤其是在 LAP 含量较高时。RSF/LAP 3D 多孔支架在大鼠体内的皮下植入实验表明具有良好的组织相容性。因此，具有良好生物相容性和可生物降解性的 RSF/LAP 3D 多孔支架在骨组织工程领域具有广阔的应用前景。
