编码小鼠二氢叶酸还原酶的DNA序列在大肠杆菌中的表型表达。

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

作者信息

Chang A C, Nunberg J H, Kaufman R J, Erlich H A, Schimke R T, Cohen S N

出版信息

Nature. 1978 Oct 19;275(5681):617-24. doi: 10.1038/275617a0.

Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

摘要

本文描述了含有哺乳动物基因序列并能表型表达该序列的细菌质粒的构建与分析。此类质粒可编码一种具有酶活性、免疫反应性以及小鼠二氢叶酸还原酶分子大小特征的蛋白质，并使宿主细胞对抗代谢药物甲氧苄啶产生抗性。