Li Shaoming, Gao Ling, Liu Jiacheng, Guo Chao, Zheng Jingjing, Zhi Keqian, Ren Wenhao
Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Qingdao University, No.1677 Wutaishan Road, Qingdao, 266555, China.
School of Stomatology of Qingdao University, Qingdao, 266003, China.
Cell Death Discov. 2022 Aug 25;8(1):373. doi: 10.1038/s41420-022-01168-1.
Autophagy is related to many cellular mechanisms and dysregulation of autophagy involves the pathological process in cancer. miR-10b activates autophagy, which promotes invasion and migration of OSCC. Its functional role in the mechanism of OSCC to autophagy remains to be unclear. Overexpression of miR-10b was followed by enhanced OSCC invasion and migration and activated autophagic protein, such as LC3II/ATG5. MiR-10b attracted Bim directly according to the Bio-informatics analyses and double luciferases reporter assays. Functional experiments further revealed that miR-10b could promote invasion and migration in vitro. In addition, miR-10b induced autophagy via inhibiting Bim in invasion and migration of OSCC. Notably, animal experiments confirmed that miR-10b-Bim promoted proliferation and autophagy in OSCC. In addition, this study provides a theoretical support for regulating the mechanism of OSCC by inducing autophagy with miR-10b-Bim as a target.
自噬与多种细胞机制相关，自噬失调涉及癌症的病理过程。miR-10b激活自噬，促进口腔鳞状细胞癌（OSCC）的侵袭和迁移。其在OSCC自噬机制中的功能作用尚不清楚。miR-10b过表达后，OSCC的侵袭和迁移增强，自噬蛋白如LC3II/ATG5被激活。根据生物信息学分析和双荧光素酶报告基因检测，miR-10b直接吸引Bim。功能实验进一步表明，miR-10b可促进体外侵袭和迁移。此外，miR-10b在OSCC的侵袭和迁移中通过抑制Bim诱导自噬。值得注意的是，动物实验证实miR-10b-Bim促进OSCC的增殖和自噬。此外，本研究为以miR-10b-Bim为靶点诱导自噬来调节OSCC机制提供了理论支持。
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