Yu Jingsheng, Zhang Wenjuan, Dao Yujie, Yang Meihua, Pang Xiaohui
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, China.
J Fungi (Basel). 2022 Aug 19;8(8):876. doi: 10.3390/jof8080876.
Fritillariae Cirrhosae Bulbus (FCB) is a well-known and precious traditional Chinese medicine with a medicinal history spanning thousands of years. In recent years, it has been reported that fungal and mycotoxin contamination influenced the safety and quality of FCB. It is essential to systematically study the fungal community for the early warning of fungal and mycotoxin contamination in this herb. A total of 15 FCB samples were collected from five provinces in China, and the fungal communities in the FCB samples were analyzed via amplifying the internal transcribed spacer 2 region through the Illumina Miseq PE300 platform. Furthermore, we compared the differences in fungal community in five groups based on collection areas. Results showed that Ascomycota (41.58-99.66%) and Mucoromycota (0-57.42%) were dominant at the phylum level. Eurotiomycetes (8.49-63.93%), Eurotiales (8.49-63.53%), and Aspergillaceae (8.49-63.51%) were the most abundant at the class, order, and family levels. (8.49-63.41%), (0-57.42%), (0-22.81%), (0.16-9.14%), and (0.06-17.95%) were the main genera in FCB samples. A total of 34 fungal taxa were identified at the species level, including five potentially toxigenic fungi namely , , , , and . The differences in fungal community between the five groups were observed. Our findings provide references for the safe utilization and quality improvement of FCB.
川贝母是一种著名且珍贵的中药材，有着数千年的药用历史。近年来，有报道称真菌和霉菌毒素污染影响了川贝母的安全性和质量。系统研究真菌群落对于该药材中真菌和霉菌毒素污染的早期预警至关重要。从中国五个省份共采集了15份川贝母样本，并通过Illumina Miseq PE300平台扩增内转录间隔区2区域，对川贝母样本中的真菌群落进行了分析。此外，我们比较了基于采集地区的五组样本中真菌群落的差异。结果表明，在门水平上，子囊菌门（41.58 - 99.66%）和毛霉门（0 - 57.42%）占主导地位。在纲、目、科水平上，散囊菌纲（8.49 - 63.93%）、散囊菌目（8.49 - 63.53%）和曲霉科（8.49 - 63.51%）最为丰富。（8.49 - 63.41%）、（0 - 57.42%）、（0 - 22.81%）、（0.16 - 9.14%）和（0.06 - 17.95%）是川贝母样本中的主要属。在种水平上共鉴定出34个真菌分类单元，包括五种潜在产毒真菌，即、、、和。观察到五组样本之间真菌群落存在差异。我们的研究结果为川贝母的安全利用和质量提升提供了参考。
