Xia GuiYan, Manawasinghe Ishara S, Phillips Alan J L, You ChunPing, Jayawardena Ruvishika S, Luo Mei, Hyde Kevin D
Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Pathogens. 2022 Jul 27;11(8):840. doi: 10.3390/pathogens11080840.
High temperatures and the seasonality in tropical ecosystems favours plant pathogens, which result in many fungal diseases. Among these, diseases caused by species are prominent as dieback, canker and leaf spots. In this research, we isolated one leaf-spot-causing species from leaves, which were collected in Guangzhou, Guangdong Province, China. Isolation and identification of the pathogen were based on morphological and molecular aspects. Based on multigene phylogenetic analysis of combined internal transcribed spacer (ITS), translation elongation factor 1-α gene () and beta-tubulin gene (), the fungus associated with leaf spots on is described as , a novel species. Pathogenicity assays were conducted by inoculating the fungus onto detached shoots and plants under controlled environmental conditions. The results revealed that the isolates can infect the plant tissues under stress conditions by developing disease symptoms on detached shoots within three days. However, when it was inoculated onto the leaves of the host and grown in natural conditions, the progression of the disease was slow. The putative pathogen was re-isolated, and Koch's assumptions were satisfied. This is the first report of species causing disease on . Results from the present study will provide additional knowledge on fungal pathogens associated with forest and ornamental plant species.
高温以及热带生态系统的季节性变化有利于植物病原体滋生,从而导致许多真菌病害。其中,由[具体物种名称]引起的病害,如枝枯病、溃疡病和叶斑病较为突出。在本研究中,我们从中国广东省广州市采集的[植物名称]叶片中分离出一种引起叶斑病的[具体物种名称]。病原体的分离和鉴定基于形态学和分子学方面。基于对内部转录间隔区(ITS)、翻译延伸因子1-α基因([具体基因名称1])和β-微管蛋白基因([具体基因名称2])组合进行的多基因系统发育分析,与[植物名称]叶斑相关的真菌被描述为[新物种名称],这是一个新物种。通过在可控环境条件下将真菌接种到离体嫩枝和植株上进行致病性测定。结果表明,[具体物种名称]分离株在胁迫条件下能够感染植物组织,在三天内使离体嫩枝出现病害症状。然而,当将其接种到寄主叶片上并在自然条件下生长时,病害发展缓慢。再次分离出假定的病原体,满足了柯赫氏法则。这是关于[具体物种名称]在[植物名称]上引起病害的首次报道。本研究结果将为与森林和观赏植物物种相关的真菌病原体提供更多知识。