Prajapati Malyaj R, Manav Aakansha, Singhal Pankhuri, Sidharthan Venkidusamy K, Sirohi Ujjwal, Kumar Mukesh, Bharti Mahesh Kumar, Singh Jitender, Kumar Pankaj, Kumar Ravindra, Prakash Satya, Baranwal Virendra Kumar
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut 250110, Uttar Pradesh, India.
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
Pathogens. 2022 Jul 30;11(8):861. doi: 10.3390/pathogens11080861.
Tuberose () is an ornamental flowering crop of the family. Tuberose mild mosaic virus (TuMMV) and tuberose mild mottle virus (TuMMoV), members of the genus , are ubiquitously distributed in most tuberose growing countries worldwide with low biological incidence. Here, we report the first coding-complete genomic RNA of TuMMV and TuMMoV obtained through high-throughput sequencing (HTS) and further, the presence of both the viruses were confirmed using virus-specific primers in RT-PCR assays. Excluding the poly (A) tail, the coding-complete genomic RNA of TuMMV and TuMMoV was 9485 and 9462 nucleotides (nts) in length, respectively, and contained a single large open reading frame (ORF). Polyprotein encoded by both the viral genomes contained nine putative cleavage sites. BLASTn analysis of TuMMV and TuMMoV genomes showed 72.40-76.80% and 67.95-77% nucleotide sequence similarities, respectively, with the existing potyviral sequences. Phylogenetic analysis based on genome sequences showed that TuMMV and TuMMoV clustered in a distinct clade to other potyviruses. Further studies are required to understand the mechanism of symptom development, distribution, genetic variability, and their possible threat to tuberose production in India.
晚香玉（）是石蒜科的一种观赏花卉作物。晚香玉轻度花叶病毒（TuMMV）和晚香玉轻度斑驳病毒（TuMMoV）属于该属成员，在全球大多数种植晚香玉的国家普遍分布，生物学发病率较低。在此，我们报告了通过高通量测序（HTS）获得的TuMMV和TuMMoV的首个编码完整基因组RNA，此外，在逆转录聚合酶链反应（RT-PCR）分析中使用病毒特异性引物证实了这两种病毒的存在。排除多聚（A）尾后，TuMMV和TuMMoV的编码完整基因组RNA长度分别为9485和9462个核苷酸（nts），并包含一个单一的大开放阅读框（ORF）。两种病毒基因组编码的多聚蛋白包含9个推定的切割位点。对TuMMV和TuMMoV基因组的BLASTn分析表明，它们与现有的马铃薯Y病毒序列的核苷酸序列相似性分别为72.40 - 76.80%和67.95 - 77%。基于基因组序列的系统发育分析表明，TuMMV和TuMMoV聚集在一个与其他马铃薯Y病毒不同的进化枝中。需要进一步研究以了解症状发展、分布、遗传变异性的机制以及它们对印度晚香玉生产可能造成的威胁。
