He Li-Yuan, Yu You-Bo, Liu Ying, Le Yao-Jin, Li Sha, Yang Xiao-Yan
Zhuhai Key Laboratory of Basic and Applied Research in Chinese Medicine, Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai, China.
Front Microbiol. 2022 Aug 9;13:969490. doi: 10.3389/fmicb.2022.969490. eCollection 2022.
is one of the main pathogenic bacteria that causes disease in humans. It is reported that over 18 million cases of disease occurred in the world, and more than 500,000 deaths occur annually worldwide. An effective vaccine is widely regarded as the most reliable way to control and prevent streptococcal infections. However, there is currently no approved vaccine for . In this study, we evaluated the potential of lipoprotein FtsB as a new vaccine candidate to prevent infection. Mice vaccinated with purified FtsB protein elicited high titers of IgG, IgG1 and IgG2a antibodies in mouse serum. Vaccinated with FtsB can reduce bacterial systemic dissemination in the blood, heart, and spleen and reduce organ damage in the mouse bacteremia model. In addition, active immunization with FtsB protected against streptococcal abscess formation. Furthermore, immunization with FtsB was efficient in inducing a mixed cellular immune response and promoting the maturation of dendritic cells in mice. The lipoprotein HtsA was served as a positive control because it has been reported to protect mice from infection in both active and passive immunization. These findings demonstrated that lipoprotein FtsB may serve as a candidate vaccine for the prevention of infection.
是导致人类发病的主要病原菌之一。据报道，全球每年有超过1800万例该疾病发生，全球每年有超过50万例死亡。一种有效的疫苗被广泛认为是控制和预防链球菌感染的最可靠方法。然而，目前尚无针对该病菌的获批疫苗。在本研究中，我们评估了脂蛋白FtsB作为预防该病菌感染的新型疫苗候选物的潜力。用纯化的FtsB蛋白免疫的小鼠在血清中诱导出高滴度的IgG、IgG1和IgG2a抗体。接种FtsB可减少小鼠菌血症模型中细菌在血液、心脏和脾脏中的全身扩散，并减少器官损伤。此外，用FtsB进行主动免疫可预防链球菌脓肿形成。此外，用FtsB免疫在诱导混合细胞免疫反应和促进小鼠树突状细胞成熟方面是有效的。脂蛋白HtsA用作阳性对照，因为据报道它在主动和被动免疫中均可保护小鼠免受该病菌感染。这些发现表明，脂蛋白FtsB可能作为预防该病菌感染的候选疫苗。
