State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming, P. R. China.
Institute of Plant Protection, Jiangsu Key Laboratory for Food Quality and Safety - State Key Laboratory Cultivation Base, Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, P. R. China.
Pest Manag Sci. 2022 Dec;78(12):5325-5333. doi: 10.1002/ps.7155. Epub 2022 Sep 12.
Plant viruses transmitted by arthropod vectors threaten crop health worldwide. Rice stripe virus (RSV) is one of the most important rice viruses in East Asia and is transmitted by the small brown planthopper (SBPH). Previously, it was demonstrated that the viral glycoprotein NSvs2-N could mediate RSV infection of the vector midgut. Therefore, NSvc2-N protein could potentially be used to reduce RSV transmission by competitively blocking midgut receptors.
Here, we report that transgenic rice plants expressing viral glycoprotein can interfere with RSV acquisition and transmission by SBPH. The soluble fraction (30-268 amino acids, designated NSvs2-N ) of NSvs2-N was transformed into rice calli, which produced plants harboring the exogenous gene. When SBPH was fed on transgenic plants prior to RSV-infected rice (sequential feeding) and when insects were fed on RSV-infected transgenic plants (concomitant feeding), virus acquisition by the insect vector was inhibited, and subsequent viral titers were reduced. Immunofluorescence labeling also indicated that viral infection of the insect midgut was inhibited after SBPH was fed on transgenic plants. The system by which RSV infected insect cells in vitro was used to corroborate the role of NSvc2-N in reducing viral infection. After the cells were incubated with transgenic rice sap, the virus infection rate of the cells decreased significantly, and viral accumulation in the cells was lower than that in the control group.
These results demonstrated the negative effect of NSvs2-N transgenic plants on RSV transmission by insect vectors, which provides a novel and effective way to control plant viral diseases. © 2022 Society of Chemical Industry.
通过节肢动物媒介传播的植物病毒威胁着全世界作物的健康。水稻条纹病毒（RSV）是东亚最重要的水稻病毒之一，由褐飞虱（SBPH）传播。此前，研究表明病毒糖蛋白 NSvs2-N 可介导 RSV 对媒介昆虫中肠的感染。因此，NSvc2-N 蛋白可能通过竞争性阻断中肠受体来减少 RSV 的传播。
本研究报道了表达病毒糖蛋白的转基因水稻可以干扰 SBPH 对 RSV 的获得和传播。将 NSvs2-N 的可溶性部分（30-268 个氨基酸，命名为 NSvs2-N）转化到水稻愈伤组织中，这些愈伤组织产生了携带外源基因的植物。当 SBPH 在 RSV 感染的水稻（序贯喂养）之前取食转基因植物，或当昆虫取食 RSV 感染的转基因植物（同时喂养）时，昆虫媒介对病毒的获得被抑制，随后病毒滴度降低。免疫荧光标记也表明，当 SBPH 取食转基因植物后，昆虫中肠的病毒感染受到抑制。体外 RSV 感染昆虫细胞的系统用于证实 NSvc2-N 在降低病毒感染中的作用。用转基因水稻汁液孵育细胞后，细胞的病毒感染率显著下降，细胞内病毒积累量低于对照组。
这些结果表明 NSvs2-N 转基因植物对昆虫媒介传播 RSV 具有负向作用，为控制植物病毒病提供了一种新颖有效的方法。 © 2022 英国化学学会。
