相邻双链 DNA 对 G-三链体-ThT 复合物荧光强度增强的影响及其在 TNOS 和 Hg 检测中的应用。

The effect of adjacent double-strand DNA on the G-triplex-ThT complex fluorescence intensity enhancement and its application in TNOS and Hg detection.

机构信息

Institute of Bionanotechnology, Ludong University, Yantai, 264025, PR China; College of Food Engineering, Ludong University, Yantai, 264025, PR China; College of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, PR China.

College of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, PR China.

出版信息

Talanta. 2023 Jan 15;252:123884. doi: 10.1016/j.talanta.2022.123884. Epub 2022 Aug 28.

DOI:10.1016/j.talanta.2022.123884

PMID:36055077

Abstract

In this paper, we have found that adjacent double-strand DNA (dsDNA) can enhance the fluorescence intensity of the G-triplex (G31)-thioflavin T (ThT) complex. By connecting the newly formed dsDNA with the G31 sequence, terminator nopaline synthase (TNOS) gene and Hg were detected. The intermolecular duplex hybridization (e.g., TNOS gene and its complementary DNA), or the intramolecular mismatched thymine (T)-Hg-T pairs induced the formation of dsDNA with planar morphology, which resulted in the increased binding capacity of adjacent G31 to ThT, as well as the enhanced fluorescence intensity of G31-ThT complex. A versatile 'turn-on' fluorescence scaffold was developed for discriminating transgenic and non-transgenic soybeans and detecting Hg in lake water.

摘要

在本文中，我们发现相邻的双链 DNA（dsDNA）可以增强 G-三链体（G31）-硫堇 T（ThT）复合物的荧光强度。通过将新形成的 dsDNA 与 G31 序列、终止子胭脂碱合成酶（TNOS）基因和汞连接起来，可以检测到。分子间双链杂交（例如，TNOS 基因与其互补 DNA）或分子内错配胸腺嘧啶（T）-汞-T 对诱导具有平面形态的 dsDNA 的形成，这导致相邻 G31 与 ThT 的结合能力增加，以及 G31-ThT 复合物的荧光强度增强。开发了一种通用的“开启”荧光支架，用于区分转基因和非转基因大豆，并检测湖水中的汞。