Chandnani Rahul, Kim Changsoo, Patel Jinesh D, Guo Hui, Shehzad Tariq, Wallace Jason G, He Daohua, Zhang Zhengsheng, Adhikari Jeevan, Khanal Sameer, Chee Peng W, Paterson Andrew H
Plant Genome Mapping Laboratory, The University of Georgia, Athens, GA, United States.
Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, South Korea.
Front Plant Sci. 2022 Aug 18;13:981682. doi: 10.3389/fpls.2022.981682. eCollection 2022.
Plant architecture, flowering time and maturity traits are important determinants of yield and fiber quality of cotton. Genetic dissection of loci determining these yield and quality components is complicated by numerous loci with alleles conferring small differences. Therefore, mapping populations segregating for smaller numbers and sizes of introgressed segments is expected to facilitate dissection of these complex quantitative traits. At an advanced stage in the development of reciprocal advanced backcross populations from crosses between elite cultivar '' (GH) and '' (GB), we undertook mapping of plant architectural traits, flowering time and maturity. A total of 284 BCF and BCF progeny rows, 120 in GH and 164 in GB background, were evaluated for phenotype, with only 4 and 3 (of 7) traits showing significant differences among progenies. Genotyping by sequencing yielded 3,186 and 3,026 SNPs, respectively, that revealed a total of 27 QTLs in GH background and 22 in GB, for plant height, days to flowering, residual flowering at maturity and maturity. More than of 90% QTLs identified in both backgrounds had small effects (%PV < 10), supporting the merit of this population structure to reduce background noise and small effect QTLs. Germplasm developed in this study may serve as potential pre-breeding material to develop improved cotton cultivars.
植株形态、开花时间和成熟性状是棉花产量和纤维品质的重要决定因素。由于众多位点的等位基因差异较小，决定这些产量和品质构成要素的基因座的遗传剖析较为复杂。因此，预期分离渗入片段数量较少且片段较小的作图群体将有助于剖析这些复杂的数量性状。在由优良品种“”（陆地棉）与“”（海岛棉）杂交构建的正反交高代回交群体的发育后期，我们对植株形态性状、开花时间和成熟度进行了作图。总共对284个BCF和BCF子代行进行了表型评估，其中120个在陆地棉背景中，164个在海岛棉背景中，子代中仅有4个和3个（共7个）性状表现出显著差异。通过测序进行基因分型分别产生了3186个和3026个单核苷酸多态性（SNP），这些SNP在陆地棉背景中总共揭示了27个数量性状基因座（QTL），在海岛棉背景中揭示了22个QTL，涉及株高、开花天数、成熟时的剩余花数和成熟度。在两个背景中鉴定出的超过90%的QTL效应较小（%PV < 10），这支持了这种群体结构在减少背景噪声和小效应QTL方面的优点。本研究中开发的种质可作为培育改良棉花品种的潜在预育种材料。
