基于验证的 QuEChERS 的 UHPLC-ESI-MS/MS 方法用于控制红色着色水果中展青霉素（真菌毒素）的污染。
Validated QuEChERS-based UHPLC-ESI-MS/MS method for the postharvest control of patulin (mycotoxin) contamination in red-pigmented fruits.
机构信息
Department of Chemistry, Institute of Biological Sciences, The John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708 Lublin, Poland.
出版信息
Food Chem. 2023 Jan 30;400:134066. doi: 10.1016/j.foodchem.2022.134066. Epub 2022 Sep 5.
The paper outlines a procedure based on ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry and QuEChERS pretreatment for the determination of patulin in selected berries and stone fruits. The mycotoxin was determined in the positive electrospray ionization mode utilizing the formation of its methanol adduct. The method was validated for 4 fruit matrices: strawberry (Rosaceae, Fragaria), raspberry (Rosaceae, Rubus), redcurrant (Rosaceae, Prunus) and sour cherry (Grossulariaceae, Ribes). The fruits were characterized by total phenolics, flavonoids, and anthocyanin contents and obtained results were within the ranges of 2.65-11.22 mg GAE/g, 1.06-4.67 mg CE/g, 0.05-1.44 mg cyd-3-glu/g, respectively. The limits of quantification ranged from 0.65 µg/kg to 3.01 µg/kg depending on the fruit type. The method was applied to 71 fruit samples collected in Poland. Analysis of moldy fruit samples has shown a high incidence of patulin contamination.
本文概述了一种基于超高效液相色谱-电喷雾电离-串联质谱和 QuEChERS 预处理的方法，用于测定选定浆果和核果中的棒曲霉素。采用甲醇加合物的形成，在正电喷雾电离模式下对真菌毒素进行了测定。该方法针对 4 种水果基质进行了验证：草莓（蔷薇科，草莓属）、覆盆子（蔷薇科，悬钩子属）、红醋栗（蔷薇科，李属）和酸樱桃（桃金娘科，穗醋栗属）。这些水果的总酚、类黄酮和花青素含量具有特征性，其含量分别为 2.65-11.22 mg GAE/g、1.06-4.67 mg CE/g 和 0.05-1.44 mg cyd-3-glu/g。根据水果类型的不同，定量限范围为 0.65 µg/kg 至 3.01 µg/kg。该方法应用于在波兰采集的 71 个水果样本。对霉变水果样本的分析表明，棒曲霉素污染的发生率很高。
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