Schiavone F M, Cooke T J
Cell Differ. 1987 Jun;21(1):53-62. doi: 10.1016/0045-6039(87)90448-9.
The effects of various exogenous auxins and polar auxin transport inhibitors on somatic embryogenesis in carrot cultures were investigated. Indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid do not disrupt the sequence or the polarity of individual stages in embryo development, but tend to cause developing embryos to revert to undifferentiated callus, with increasing frequency in later embryo stages. The transport inhibitors, N-(1-naphthyl)phthalamic acid and 2,3,5-triiodobenzoic acid, block morphological transitions to the subsequent stage; for example, they cause the formation of enlarged globular and oblong embryos. Heart embryos in these treatments usually develop additional lateral growth axes. These results shed light on the role of auxin and its polar transport in somatic embryogenesis.
研究了各种外源生长素和极性生长素运输抑制剂对胡萝卜培养物体细胞胚胎发生的影响。吲哚-3-乙酸和2,4-二氯苯氧乙酸不会破坏胚胎发育各个阶段的顺序或极性，但往往会导致发育中的胚胎恢复为未分化的愈伤组织，且在胚胎发育后期这种情况出现的频率增加。运输抑制剂N-（1-萘基）邻苯二甲酸和2,3,5-三碘苯甲酸会阻断向后续阶段的形态转变；例如，它们会导致形成增大的球形和椭圆形胚胎。在这些处理中，心形胚通常会发育出额外的侧向生长轴。这些结果揭示了生长素及其极性运输在体细胞胚胎发生中的作用。
