Kadokawa Y, Kato Y, Eguchi G
Cell Differ. 1987 Jun;21(1):69-76. doi: 10.1016/0045-6039(87)90450-7.
Cell lineages of the primitive endoderm and the visceral endoderm of mouse embryos were examined by culturing whole embryos in vitro. The primitive endoderm and visceral endoderm cells could be labelled by incubation of embryos in a medium containing horse radish peroxidase (HRP). HRP localization was chased throughout the culture period. The results show that the visceral endoderm derives from the primitive endoderm, and the visceral endoderm forms only the extra-embryonic endoderm (yolk sac endoderm) of the conceptus. The definitive endoderm which is probably derived from the head process, newly appears on the ventral surface of the embryo.
通过体外培养完整胚胎，研究了小鼠胚胎原始内胚层和脏内胚层的细胞谱系。将胚胎在含有辣根过氧化物酶（HRP）的培养基中孵育，可标记原始内胚层和脏内胚层细胞。在整个培养期间追踪HRP的定位。结果表明，脏内胚层起源于原始内胚层，且脏内胚层仅形成孕体的胚外内胚层（卵黄囊内胚层）。可能源自头突的定形内胚层新出现在胚胎的腹面。
