Zhang Jinhao, Ahmed Waqar, Zhou Xinghai, Yao Bo, He Zulei, Qiu Yue, Wei Fangjun, He Yilu, Wei Lanfang, Ji Guanghai
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
Key Laboratory of Agro-Biodiversity and Pest Management of Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
Plants (Basel). 2022 Sep 2;11(17):2295. doi: 10.3390/plants11172295.
Clubroot caused by is an economically important soilborne disease of Chinese cabbage worldwide. Integrated biological control through crop rotation is considered a good disease management approach to suppress the incidence of soilborne diseases. In this study, we evaluated the effect of a marigold plant (root exudates, crude extract, and powder) on the germination and death of resting spores of in vitro assays. Additionally, we also performed 16S high throughput sequencing, to investigate the impact of marigold-Chinese cabbage crop rotation on soil bacterial community composition, to manage this devastating pathogen. This study revealed that the marigold root exudates, crude extract, and powder significantly promoted the germination and death of resting spores. Under field conditions, marigold-Chinese cabbage crop rotation with an empty period of at least 15 days enhanced the germination of resting spores, shifted the rhizosphere bacterial community composition, and suppressed the incidence of clubroot by up to 63.35%. Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, and Verrucomicrobia were the most dominant phyla and were present at high relative levels in the rhizosphere soil of Chinese cabbage. We concluded that crop rotation of Chinese cabbage with marigold can significantly reduce the incidence of clubroot disease in the next crop. To our knowledge, this is the first comprehensive study on the prevention and control of clubroot disease in Chinese cabbage through crop rotation with marigold.
由[病原体名称未给出]引起的根肿病是全球范围内对大白菜具有重要经济影响的土传病害。通过作物轮作进行综合生物防治被认为是抑制土传病害发生率的一种良好病害管理方法。在本研究中，我们在体外试验中评估了万寿菊植株（根系分泌物、粗提物和粉末）对[病原体名称未给出]休眠孢子萌发和死亡的影响。此外，我们还进行了16S高通量测序，以研究万寿菊 - 大白菜轮作对土壤细菌群落组成的影响，从而防治这种毁灭性病原体。本研究表明，万寿菊根系分泌物、粗提物和粉末显著促进了[病原体名称未给出]休眠孢子的萌发和死亡。在田间条件下，间隔至少15天的万寿菊 - 大白菜轮作提高了[病原体名称未给出]休眠孢子的萌发率，改变了根际细菌群落组成，并将根肿病的发病率降低了多达63.35%。变形菌门、酸杆菌门、拟杆菌门、放线菌门和疣微菌门是最主要的门类，在大白菜根际土壤中相对含量较高。我们得出结论，大白菜与万寿菊轮作可显著降低下茬作物根肿病的发病率。据我们所知，这是第一项关于通过与万寿菊轮作来防治大白菜根肿病的综合研究。
