Department of Obstetrics and Gynecology, Binhai County People's Hospital, Jiangsu, China.
Rev Invest Clin. 2022;74(4):181-192. doi: 10.24875/RIC.22000137.
Cervical cancer (CC), as a serious menace to the health of women, has long been one of the most lethal gynecologic neoplasms throughout the world. Long non-coding RNA (LncRNA) has been documented to exert crucial functions in many malignant tumors. Nonetheless, the function and molecular mechanism of in CC remain completely unknown.
This study aimed to explore the function and molecular mechanism of in CC.
The expression levels of NR2F1-AS1, miR-642a-3p, NR2F1 in CC tissues, and cell lines were examined by reverse transcription real-time quantitative polymerase chain reaction. Cell viability, proliferation, migration, and invasion were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, colony formation and Transwell assays. The protein levels of epithelial-mesenchymal transition markers and NR2F1 in CC cells were assessed by Western blot analysis. The correlations among NR2F1-AS1, miR-642a-3p, and NR2F1 were estimated through luciferase reporter and RNA immunoprecipitation assays.
1 expression was clearly downregulated in CC tissues and cell lines. Molecular mechanistic experiments showed that NR2F1-AS1 overexpression upregulated NR2F1 expression in CC cells by directly binding to miR-642a-3p, and inhibiting by this way cell viability, proliferation, migration, and invasion in CC. Rescue assays showed that NR2F1 knockdown or miR-642a-3p overexpression offset NR2F1-AS1 upregulation-induced inhibition on CC cell malignant phenotypes.
These findings revealed that NR2F1-AS1 played a tumor suppressor role in CC by mediating the miR-642a-3p/NR2F1 axis.
宫颈癌（CC）作为对女性健康的严重威胁，长期以来一直是全世界最致命的妇科肿瘤之一。长链非编码 RNA（LncRNA）已被证明在许多恶性肿瘤中发挥着至关重要的作用。然而，在 CC 中，的功能和分子机制仍完全未知。
本研究旨在探讨在 CC 中的功能和分子机制。
通过逆转录实时定量聚合酶链反应检测 CC 组织和细胞系中 NR2F1-AS1、miR-642a-3p 和 NR2F1 的表达水平。通过 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴盐、集落形成和 Transwell 测定检测细胞活力、增殖、迁移和侵袭。通过 Western blot 分析评估 CC 细胞中上皮-间充质转化标志物和 NR2F1 的蛋白水平。通过荧光素酶报告和 RNA 免疫沉淀测定评估 NR2F1-AS1、miR-642a-3p 和 NR2F1 之间的相关性。
1 在 CC 组织和细胞系中明显下调。分子机制实验表明，NR2F1-AS1 通过直接结合 miR-642a-3p 上调 CC 细胞中的 NR2F1 表达，从而抑制 CC 细胞的活力、增殖、迁移和侵袭。挽救实验表明，NR2F1 敲低或 miR-642a-3p 过表达抵消了 NR2F1-AS1 上调诱导的对 CC 细胞恶性表型的抑制作用。
这些发现表明，NR2F1-AS1 通过介导 miR-642a-3p/NR2F1 轴在 CC 中发挥肿瘤抑制作用。
