Wang Fangling, Lv Wenjuan, Zhang Yanli, Niu Xiao, Wu Xuequan, Chen Hongli, Chen Xingguo
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
J Chromatogr A. 2022 Oct 11;1681:463463. doi: 10.1016/j.chroma.2022.463463. Epub 2022 Aug 31.
Covalent organic frameworks (COFs) are divided into two-dimensional (2D) and three-dimensional (3D) structures according to the connection dimension of covalent bonds. 3D COFs have smaller pore size and larger surface area, which would facilitate the separation of small organic molecules with similar structures and properties in capillary electrochromatographic (CEC) separation. However, the application of 3D COFs in CEC is still in its early stages. Thus, 3D COFs (3D-IL-COF-1 and 3D-IL-COF-2) were synthesis by a new synthesis method in this work. The related characterization results confirmed that spherical 3D-IL-COFs formed by the accumulation of small polyhedral particles were successfully synthesized. Then, 3D-IL-COFs were introduced into the inner wall of capillary by in-situ growth method to prepare the 3D COFs coated capillaries for the first time. The electrochromatographic separation performance of the 3D-IL-COFs coated capillaries was investigated using the 3D-IL-COF-1 coated capillary as the model. The results indicate that the 3D-IL-COF-1 coated capillary with uniform coating exhibits a broad-spectrum separation capacity not only for acidic, basic and neutral analytes but also for neutral isomers, which shows that 3D-IL-COFs becomes an attractive potential stationary phase for electrochromatographic separation.
共价有机框架（COFs）根据共价键的连接维度分为二维（2D）和三维（3D）结构。三维COFs具有较小的孔径和较大的表面积，这将有助于在毛细管电色谱（CEC）分离中分离具有相似结构和性质的小有机分子。然而，三维COFs在CEC中的应用仍处于早期阶段。因此，本工作采用一种新的合成方法合成了三维COFs（3D-IL-COF-1和3D-IL-COF-2）。相关表征结果证实成功合成了由小多面体颗粒堆积形成的球形三维IL-COFs。然后，通过原位生长法将三维IL-COFs引入毛细管内壁，首次制备了三维COFs涂层毛细管。以3D-IL-COF-1涂层毛细管为模型，研究了3D-IL-COFs涂层毛细管的电色谱分离性能。结果表明，具有均匀涂层的3D-IL-COF-1涂层毛细管不仅对酸性、碱性和中性分析物具有广谱分离能力，而且对中性异构体也具有分离能力，这表明3D-IL-COFs成为一种有吸引力的电色谱分离潜在固定相。
