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在对单个胶带提取残片上收集的 GSR 和 DNA 痕迹进行连续法医分析方面的经验。

Experiences in successive forensic analysis of GSR and DNA traces collected on a single tape lift stub.

机构信息

Netherlands Forensic Institute, Gunshot Residue group, The Hague, The Netherlands.

Netherlands Forensic Institute, Division of Biological Traces, The Hague, The Netherlands.

出版信息

J Forensic Sci. 2022 Nov;67(6):2409-2415. doi: 10.1111/1556-4029.15121. Epub 2022 Sep 11.

Abstract

Gunshot residue (GSR) and deoxyribonucleic acid (DNA) analyses are used in the reconstruction of shooting incidents involving firearms. In both fields, adhesive tapes are commonly applied to recover traces. Using a simultaneous sampling approach for retrieving both types of traces from objects related to a shooting can be powerful. We present the results for DNA testing and GSR analysis using "DNA stubs" to recover both types of traces in one sampling. The analyses are successive: stubs are first examined with scanning electron microscopy for the presence of GSR and next subjected to DNA extraction and short tandem repeat (STR) typing. The results show no negative effect on the DNA and GSR results. The risk of DNA contamination during GSR analysis is addressed, showing that it is negligible. Our experiences in casework show that the approach can be successfully implemented.

摘要

枪击残留物(GSR)和脱氧核糖核酸(DNA)分析用于重建涉及枪支的枪击事件。在这两个领域,通常使用胶带来回收痕迹。从与枪击事件相关的物体上同时采集这两种痕迹可以提供有力的证据。我们介绍了使用“DNA 短段”从一种采样中同时回收这两种痕迹的 DNA 检测和 GSR 分析的结果。分析是连续进行的:先用扫描电子显微镜检查短段中是否存在 GSR,然后进行 DNA 提取和短串联重复(STR)分型。结果显示,这对 DNA 和 GSR 结果没有负面影响。还讨论了 GSR 分析过程中 DNA 污染的风险,结果表明风险可以忽略不计。我们在案例工作中的经验表明,该方法可以成功实施。

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