Rapid detection of based on a new target gene .

causes crown and root wilting in more than 5,000 plant species and represents a significant threat to the health of natural ecosystems and horticultural crops. The early and accurate detection of is a fundamental step in disease prevention and appropriate management. In this study, based on public genomic sequence data and bioinformatic analysis of several , , and species, we have identified a new target gene, Pcinn13739; this allowed us to establish a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay for the detection of . -RPA-LFD assay was highly specific to . Test results for 12 isolates of were positive, but negative for 50 isolates of 25 kinds of  species, 13 isolates of 10 kinds of  and  species, 32 isolates of 26 kinds of fungi species, and 11 isolates of two kinds of species. By detecting as little as 10 pg.µl of genomic DNA from in a 50-µl reaction, the RPA-LFD assay was 100 times more sensitive than conventional PCR assays. By using RPA-LFD assay, was also detected on artificially inoculated fruit from , the leaves of , the roots of sterile , and the artificially inoculated soil. Results in this study indicated that this sensitive, specific, and rapid RPA-LFD assay has potentially significant applications to diagnosing , especially under time- and resource-limited conditions.