Department of Civil Engineering, GLA University, Mathura, Uttar Pradesh 281406, India.
Department of Computer Science Engineering, MIT Art, Design and Technology University, Pune, Maharashtra 412201, India.
Comput Intell Neurosci. 2022 Sep 1;2022:4411876. doi: 10.1155/2022/4411876. eCollection 2022.
The focus of this research is to isolating and identifying bacteria that produce calcite precipitate, as well as determining whether or not these bacteria are suitable for incorporation into concrete in order to enhance the material's strength and make the environment protection better. In order to survive the high "potential of hydrogen" of concrete, microbes that are going to be added to concrete need to be able to withstand alkali, and they also need to be able to develop endospores so that they can survive the mechanical forces that are going to be put on the concrete while it is being mixed. In order to precipitate CaCO in the form of calcite, they need to have a strong urease activity. Both Bacillus sphaericus and the Streptococcus aureus bacterial strains were evaluated for their ability to precipitate calcium carbonate (CaCO). These strains were obtained from the Department of Biotechnology at GLA University in Mathura. This research aims to solve the issue of augmenting the tension and compression strengths of concrete by investigating possible solutions for environmentally friendly concrete. The sterile cultures of the microorganisms were mixed with water, which was one of the components of the concrete mixture, along with the nutrients in the appropriate proportions. After that, the blocks were molded, and then pond-cured for 7, 28, 56, 90, 120, 180, 270, and 365 days, respectively, before being evaluated for compressibility and tensile strength. An investigation into the effect that bacteria have on compression strength was carried out, and the outcomes of the tests showed that bacterial concrete specimens exhibited an increase in mechanical strength. When compared to regular concrete, the results showed a maximum increase of 16 percent in compressive strength and a maximum increase of 12 percent in split tensile strength. This study also found that both bacterial concrete containing 106, 107, and 108 cfu/ml concentrations made from Bacillus sphaericus and Streptococcus aureus bacteria gave better results than normal concrete. Both cluster analysis (CA) and regression analysis (RA) were utilized in this research project in order to measure and analyze mechanical strength.
本研究的重点是分离和鉴定产生方解石沉淀的细菌，并确定这些细菌是否适合掺入混凝土中，以提高材料的强度，更好地保护环境。为了在混凝土的高“氢潜力”下生存，将要添加到混凝土中的微生物需要能够耐受碱，并且它们还需要能够形成芽孢，以便在混凝土混合时能够承受机械力。为了以方解石的形式沉淀 CaCO，它们需要具有很强的脲酶活性。评估了球形芽孢杆菌和金黄色葡萄球菌两种细菌菌株沉淀碳酸钙（CaCO）的能力。这些菌株是从马图拉 GLA 大学生物技术系获得的。本研究旨在通过研究环保型混凝土的可能解决方案来解决提高混凝土的拉伸和压缩强度的问题。将无菌培养的微生物与水混合，水是混凝土混合物的成分之一，以及适当比例的营养物质。然后，将块模制，然后分别在池塘中养护 7、28、56、90、120、180、270 和 365 天，然后分别评估其压缩性和拉伸强度。研究了细菌对压缩强度的影响，测试结果表明细菌混凝土试件的机械强度增加。与普通混凝土相比，结果显示抗压强度最大增加 16%，劈裂拉伸强度最大增加 12%。本研究还发现，含有 106、107 和 108 cfu/ml 浓度的细菌混凝土，由球形芽孢杆菌和金黄色葡萄球菌制成，比普通混凝土效果更好。聚类分析（CA）和回归分析（RA）都用于本研究项目，以测量和分析机械强度。
