Jin Tao, An Qingzhu, Qin Xuanfeng, Qin Xuanfeng, Hu Yuanyuan, Hu Jia, Zhou Bing, Leng Bing
Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
Acta Biochim Pol. 2022 Sep 13;69(3):613-618. doi: 10.18388/abp.2020_5982.
Cerebral aneurysm (CA) is one of the most common cerebrovascular diseases. The study was conducted to investigate the effect of resveratrol (RES) on the CA formation and its possible mechanisms.
Murine model of CA was constructed by induced hypertension and fed without (model group) or with RES (RES group). A Sham group was used as a control. The CA formation and inflammatory response were examined morphologically and histochemically. The expression of nuclear factor-κB (NF-κB), matrix metalloproteinase (MMP)-2, and MMP-9 was analyzed using qRT-PCR and Western blots.
CA was induced in mice after the left common carotid artery was ligated and fed with high sodium chloride. Compared with the model, mice fed with RES had significantly fewer CA with smaller size, normal thickness of the arterial wall (P<0.05), and fewer infiltrated macrophages in the aneurysm wall (P<0.05). qRT-PCR and Western blot analyses showed that the expression of MMP-2, MMP-9 and NF-κB was significantly elevated in the model as compared with the control and significantly decreased after RES treatments (P<0.05).
RES can inhibit the CA formation in mice subjected to induced hypertension and this inhibition is likely mediated via downregulating the NF-κB pathway.
脑动脉瘤（CA）是最常见的脑血管疾病之一。本研究旨在探讨白藜芦醇（RES）对CA形成的影响及其可能机制。
通过诱导高血压构建CA小鼠模型，分为不给予RES（模型组）和给予RES（RES组）喂养，设假手术组作为对照。通过形态学和组织化学方法检测CA形成及炎症反应。采用qRT-PCR和蛋白质免疫印迹法分析核因子-κB（NF-κB）、基质金属蛋白酶（MMP）-2和MMP-9的表达。
结扎小鼠左颈总动脉并给予高氯化钠喂养后诱导出CA。与模型组相比，RES喂养的小鼠CA数量显著减少、尺寸更小，动脉壁厚度正常（P<0.05），动脉瘤壁内浸润的巨噬细胞数量更少（P<0.05）。qRT-PCR和蛋白质免疫印迹分析显示，与对照组相比，模型组中MMP-2、MMP-9和NF-κB的表达显著升高，RES处理后显著降低（P<0.05）。
RES可抑制诱导高血压小鼠的CA形成，这种抑制作用可能是通过下调NF-κB通路介导的。
