采用液相色谱-串联质谱法测定尿游离皮质醇和皮质酮。

Measurement of Urinary Free Cortisol and Cortisone by LC-MS/MS.

作者信息

Ray Julie A, Kish-Trier Erik, Johnson Lisa M

机构信息

ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.

Department of Pathology, University of Utah, Salt Lake City, UT, USA.

出版信息

Methods Mol Biol. 2022;2546:119-128. doi: 10.1007/978-1-0716-2565-1_11.

DOI:10.1007/978-1-0716-2565-1_11

PMID:36127583

Abstract

Monitoring urinary free cortisol (UFC) excretion helps assess adrenal function and is used to screen for endogenous Cushing's syndrome caused by an adrenal or pituitary tumor. While serum cortisol levels fluctuate in response to time of day, stress, and concentrations of cortisol-binding globulin (CBG), a 24-h urine collection measures the cortisol produced over the entire day and does not suffer from as much variability as a serum measurement.We describe here a method of measurement of urinary free cortisol (UFC) and cortisone using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Urine samples, combined with stable isotope-labeled internal standards, are extracted by liquid-liquid extraction using ethyl acetate and hexane. An API 5500 mass spectrometer operated in positive atmospheric pressure chemical ionization (APCI) mode is used for detection.

摘要

监测尿游离皮质醇（UFC）排泄有助于评估肾上腺功能，并用于筛查由肾上腺或垂体肿瘤引起的内源性库欣综合征。虽然血清皮质醇水平会因一天中的时间、压力和皮质醇结合球蛋白（CBG）浓度而波动，但24小时尿液收集可测量一整天产生的皮质醇，并且不像血清测量那样存在那么大的变异性。我们在此描述一种使用液相色谱 - 串联质谱法（LC-MS/MS）测量尿游离皮质醇（UFC）和可的松的方法。将尿液样本与稳定同位素标记的内标物混合，通过使用乙酸乙酯和己烷的液 - 液萃取进行提取。使用在正大气压化学电离（APCI）模式下运行的API 5500质谱仪进行检测。