Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
Biomolecules. 2022 Sep 3;12(9):1233. doi: 10.3390/biom12091233.
SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urine of 308 COVID-19 patients using liquid chromatography-mass spectrometry (LC-MS). We found that two modified nucleosides, -threonylcarbamoyladenosine (tA) and 2-methylthio--threonylcarbamoyladenosine (mstA), were elevated in serum and urine of COVID-19 patients. Moreover, these levels were associated with symptom severity and decreased upon recovery from COVID-19. In addition, the elevation of similarly modified nucleosides was observed regardless of COVID-19 variants. These findings illuminate specific modified RNA nucleosides in the extracellular fluids as biomarkers for COVID-19 infection and severity.
SARS-CoV-2 感染会改变细胞内的 RNA 含量。细胞 RNA 会发生化学修饰并最终降解，将修饰后的核苷沉积到血清和尿液等细胞外液中。在这里，我们使用液相色谱-质谱法 (LC-MS) 检测了 308 例 COVID-19 患者血清和尿液中含有的修饰核苷水平的 COVID-19 特异性变化。我们发现，两种修饰核苷，-苏氨酰氨基甲酰腺苷 (tA) 和 2-甲基硫代-苏氨酰氨基甲酰腺苷 (mstA)，在 COVID-19 患者的血清和尿液中升高。此外，这些水平与症状严重程度相关，并在 COVID-19 康复后降低。此外，无论 COVID-19 变体如何，都观察到类似修饰核苷的升高。这些发现阐明了细胞外液中特定修饰的 RNA 核苷作为 COVID-19 感染和严重程度的生物标志物。
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