Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 18071 Granada, Spain.
Institute of Nutrition and Food Technology "José Mataix", Biomedical Research Center, University of Granada, 18016 Armilla, Spain.
Int J Mol Sci. 2022 Sep 16;23(18):10813. doi: 10.3390/ijms231810813.
Previous works have described the activity of subsp. CECT 7210 (also commercially named IM-1) against rotavirus in mice and intestinal pathogens in piglets, as well as its diarrhea-reducing effect on healthy term infants. In the present work, we focused on the intestinal immunomodulatory effects of IM-1 and for this purpose we used the epithelial cell line isolated from colorectal adenocarcinoma Caco-2 and a co-culture system of human dendritic cells (DCs) from peripheral blood together with Caco-2 cells. Single Caco-2 cultures and Caco-2: DC co-cultures were incubated with IM-1 or its supernatant either in the presence or absence of CECT 515. The IM-1 supernatant exerted a protective effect against the cytotoxicity caused by CECT 515 on single cultures of Caco-2 cells as viability reached the values of untreated cells. IM-1 and its supernatant also decreased the secretion of pro-inflammatory cytokines by Caco-2 cells and the co-cultures incubated in the presence of CECT 515, with the response being more modest in the latter, which suggests that DCs modulate the activity of Caco-2 cells. Overall, the results obtained point to the immunomodulatory activity of this probiotic strain, which might underlie its previously reported beneficial effects.
先前的研究已经描述了亚属 CECT 7210（也称为 IM-1）在小鼠轮状病毒和仔猪肠道病原体中的活性，以及其对健康足月婴儿腹泻的减少作用。在本研究中，我们专注于 IM-1 的肠道免疫调节作用，为此我们使用了源自结直肠腺癌 Caco-2 的上皮细胞系和人外周血树突状细胞（DC）的共培养系统与 Caco-2 细胞一起。单独的 Caco-2 培养物和 Caco-2：DC 共培养物与 IM-1 或其上清液孵育，无论是否存在 CECT 515。IM-1 上清液对 CECT 515 对 Caco-2 细胞单培养物的细胞毒性具有保护作用，因为活力达到未处理细胞的水平。IM-1 和其上清液还降低了 Caco-2 细胞和在 CECT 515 存在下孵育的共培养物中促炎细胞因子的分泌，在后一种情况下反应更为温和，这表明 DC 调节 Caco-2 细胞的活性。总的来说，这些结果表明该益生菌菌株具有免疫调节活性，这可能是其先前报道的有益作用的基础。
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