Wang Huiru, Liu Huilan, Zhou Li, Wang Dongyao, Wang Shushu, Liu Qian, Wu Yun, Tu Meijuan, Sun Zimin, Zheng Xiaohu, Fu Binqing, Wang Baolong, Wei Haiming
Department of Transfusion, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China.
Institute of Immunology, University of Science and Technology of China, Hefei 230027, China.
iScience. 2022 Sep 5;25(10):105065. doi: 10.1016/j.isci.2022.105065. eCollection 2022 Oct 21.
Cytomegalovirus (CMV) infection remains one of the most frequent and life-threatening infectious complications after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Herein, we comprehensively compared the immune cells of patients with uncontrolled and controlled CMV infection post-allo-HSCT and found that B-cells were extraordinarily insufficient because of impaired B-cells reconstitution in the uncontrolled infection group. Furthermore, in the controlled infection group, reconstructed B-cells showed signatures of mature B-cells, high expression of and , and enrichment of CMV-associated B-cell receptors, which were lacking in the uncontrolled infection group. Consistently, sera from the uncontrolled infection group failed to inhibit CMV infection via neutralizing virus because of its lower content of anti-CMV-specific immunoglobulin G (IgG) than the controlled infection group. Overall, these results highlighted the contribution of B cells and anti-CMV-specific neutralizing IgGs to the restraint of CMV infection post-allo-HSCT, suggesting their potential as a supplementary treatment to improve outcomes.
巨细胞病毒（CMV）感染仍然是异基因造血干细胞移植（allo-HSCT）后最常见且危及生命的感染并发症之一。在此，我们全面比较了allo-HSCT后CMV感染未得到控制和已得到控制的患者的免疫细胞，发现未得到控制的感染组中B细胞极度不足，原因是B细胞重建受损。此外，在感染得到控制的组中，重建的B细胞表现出成熟B细胞的特征， 和 的高表达，以及CMV相关B细胞受体的富集，而这些在未得到控制的感染组中是缺乏的。同样，未得到控制的感染组的血清由于其抗CMV特异性免疫球蛋白G（IgG）含量低于感染得到控制的组，无法通过中和病毒来抑制CMV感染。总体而言，这些结果突出了B细胞和抗CMV特异性中和IgG对allo-HSCT后抑制CMV感染的作用，表明它们作为改善治疗结果的辅助治疗手段的潜力。
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