McMaster Ancient DNA Centre, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada; Department of Anthropology, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada.
McMaster Ancient DNA Centre, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada; Department of Anthropology, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada.
Int J Paleopathol. 2022 Dec;39:20-34. doi: 10.1016/j.ijpp.2022.08.002. Epub 2022 Sep 26.
To investigate variation in ancient DNA recovery of Brucella melitensis, the causative agent of brucellosis, from multiple tissues belonging to one individual MATERIALS: 14 samples were analyzed from the mummified remains of the Blessed Sante, a 14 century Franciscan friar from central Italy, with macroscopic diagnosis of probable brucellosis.
Shotgun sequencing data from was examined to determine the presence of Brucella DNA.
Three of the 14 samples contained authentic ancient DNA, identified as belonging to B. melitensis. A genome (23.81X depth coverage, 0.98 breadth coverage) was recovered from a kidney stone. Nine of the samples contained reads classified as B. melitensis (7-169), but for many the data quality was insufficient to withstand our identification and authentication criteria.
We identified significant variation in the preservation and abundance of B. melitensis DNA present across multiple tissues, with calcified nodules yielding the highest number of authenticated reads. This shows how greatly sample selection can impact pathogen identification.
Our results demonstrate variation in the preservation and recovery of pathogen DNA across tissues. This study highlights the importance of sample selection in the reconstruction of infectious disease burden and highlights the importance of a holistic approach to identifying disease.
Study focuses on pathogen recovery in a single individual.
Further analysis of how sampling impacts aDNA recovery will improve pathogen aDNA recovery and advance our understanding of disease in past peoples.
调查从一个个体的多个组织中回收布鲁氏菌属（引起布鲁氏菌病的病原体）的古代 DNA 的变化。
分析了来自意大利中部的 14 世纪方济各会修士 Blessed Sante 的 14 个木乃伊化遗体样本，这些样本的宏观诊断为可能患有布鲁氏菌病。
检查了来自 shotgun 测序的数据，以确定是否存在布鲁氏菌 DNA。
14 个样本中的 3 个包含真实的古代 DNA，鉴定为属于布鲁氏菌属。从肾结石中恢复了一个基因组（23.81X 深度覆盖，0.98 广度覆盖）。9 个样本中含有被归类为布鲁氏菌属（7-169）的读数，但对于许多样本，数据质量不足以承受我们的识别和验证标准。
我们发现，在多个组织中，布鲁氏菌属 DNA 的保存和丰度存在显著差异，钙化结节产生了最多数量的经认证的读数。这表明样本选择对病原体鉴定的影响有多大。
我们的结果表明，病原体 DNA 在组织中的保存和恢复存在差异。本研究强调了在重建传染病负担时样本选择的重要性，并强调了采用整体方法识别疾病的重要性。
研究集中于单个个体中病原体的恢复。
进一步分析采样如何影响 aDNA 恢复将改善病原体 aDNA 恢复，并提高我们对过去人群疾病的理解。
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