Shenzhen Institute of Advanced Technology (SIAT), Chinese Academy of Sciences (CAS), Shenzhen, People's Republic of China.
Department of Otolaryngology, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.
Methods Mol Biol. 2023;2559:137-149. doi: 10.1007/978-1-0716-2647-4_10.
Ubiquitin, a 76-amino acid protein which could covalently bind to a target protein, is catalyzed by an enzymatic cascade comprising of ATP-dependent ubiquitin-activating enzyme E1, ubiquitin-conjugating enzyme E2, and ubiquitin-protein ligase E3. Histidine (His) pull-down and immunoprecipitation (IP) assays are frequently adopted to detect FOXP3 ubiquitination, which is one of the most important posttranslational modifications and may induce signal transduction or 26S proteasome-dependent degradation of FOXP3 in various immune milieus.
泛素是一种 76 个氨基酸的蛋白质，可以通过一个由 ATP 依赖性泛素激活酶 E1、泛素结合酶 E2 和泛素蛋白连接酶 E3 组成的酶级联反应共价结合到靶蛋白上。组氨酸（His）下拉和免疫沉淀（IP）实验常用于检测 FOXP3 的泛素化，这是最重要的翻译后修饰之一，可能在各种免疫环境中诱导 FOXP3 的信号转导或 26S 蛋白酶体依赖性降解。
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