Université de Paris, Institut Cochin, INSERM, CNRS, Paris, France.
Department of Anatomy, Kyorin University Faculty of Medicine, Tokyo, Japan.
Methods Mol Biol. 2022;2550:207-218. doi: 10.1007/978-1-0716-2593-4_26.
The melatonin receptor subfamily belongs to the G protein-coupled receptor superfamily and consists of three members in mammals, MT, MT, and GPR50. These receptors can interact with each other to form homo- and heterodimers that are part of larger molecular complexes composed of G proteins, β-arrestins, and other membrane and cytosolic proteins. BRET (bioluminescence resonance energy transfer) is a versatile technique to follow protein-protein interactions on the nanometer scale, in real time, in living cells, which contributed largely to our understanding of the function of melatonin receptors. In this chapter, we describe our BRET protocols for melatonin receptors, which can also be applied to other GPCRs.
褪黑素受体亚家族属于 G 蛋白偶联受体超家族，哺乳动物中有 3 个成员，即 MT1、MT2 和 GPR50。这些受体可以相互作用，形成同型和异型二聚体，这些二聚体是由 G 蛋白、β-arrestin 和其他膜和胞质蛋白组成的更大分子复合物的一部分。BRET（生物发光共振能量转移）是一种在纳米尺度上实时跟踪活细胞中蛋白质-蛋白质相互作用的多功能技术，这在很大程度上促进了我们对褪黑素受体功能的理解。在本章中，我们描述了用于褪黑素受体的 BRET 方案，该方案也可应用于其他 GPCR。
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