Division of Neuroscience, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Vita-Salute San Raffaele University, Milan, Italy.
Methods Mol Biol. 2022;2550:453-461. doi: 10.1007/978-1-0716-2593-4_44.
Melatonin (MLT) has been implicated in several pathophysiological states, including pain. MLT mostly activates two G protein-coupled receptors, MT and MT. MLT displays analgesic properties in several animal paradigms of acute, inflammatory, and neuropathic pain. Although the analgesic mechanism of action of MLT is not yet completely elucidated, there is strong preclinical evidence suggesting the pharmacological potential of melatonergic compounds for treating pain. Importantly, MLT and melatonergic compounds seem to have a favorable toxicological profile than currently approved analgesic drugs. These compounds may thus deserve to be further developed as novel analgesic drugs, but this process relies on the use of appropriate and standardized experimental procedures. Therefore, in this chapter, we present the methodology to study the analgesic properties of MLT and melatonergic drugs in a preclinical model of chronic and acute pain. In addition to technical details of the surgical technique, details of anesthesia and perioperative care are also included.
褪黑素 (MLT) 与多种病理生理状态有关，包括疼痛。MLT 主要激活两种 G 蛋白偶联受体，MT 和 MT。MLT 在几种急性、炎症和神经性疼痛的动物模型中表现出镇痛特性。尽管 MLT 的镇痛作用机制尚未完全阐明，但有强有力的临床前证据表明，褪黑素化合物治疗疼痛具有潜在的药理学作用。重要的是，MLT 和褪黑素化合物的毒理学特征似乎优于目前批准的镇痛药。因此，这些化合物可能值得进一步开发为新型镇痛药，但这一过程依赖于使用适当和标准化的实验程序。因此，在本章中，我们介绍了在慢性和急性疼痛的临床前模型中研究 MLT 和褪黑素类药物镇痛特性的方法。除了手术技术的技术细节外，还包括麻醉和围手术期护理的详细信息。
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