Gabig T G, Bearman S I, Babior B M
Blood. 1979 Jun;53(6):1133-9.
The respiratory burst of human neutrophils was measured under conditions of hypoxia and low pH. O2 -- production by neutrophils activated with opsonized zymosan fell slowly as the oxygen concentration declined to 1%, then dropped more sharply, reaching negligible levels at oxygen concentrations less than 0.25%. Production was half maximal at an oxygen concentration of 0.35% (equivalent to approximately 10-microM dissolved oxygen). O2- production by the cell-free O2- -forming system prepared from zymosan-activated neutrophils showed a similar dependence on oxygen concentration. A drop in pH caused decreases in both oxygen consumption and O2-- production by zymosan-treated neutrophils, values at PH 6.0 being 10%--20% of those observed at pH 7.5. Experiments with the cell-free O2-- -forming system suggested that this decline in respiratory burst activity at low pH was due to inefficient activation of the O2-- -forming enzyme under acidic conditions.
在缺氧和低pH条件下测定了人中性粒细胞的呼吸爆发。随着氧浓度降至1%，经调理酵母聚糖激活的中性粒细胞产生O2-的速率缓慢下降，然后下降得更为急剧，在氧浓度低于0.25%时达到可忽略不计的水平。在氧浓度为0.35%（相当于约10微摩尔溶解氧）时，产生量达到最大值的一半。由酵母聚糖激活的中性粒细胞制备的无细胞O2-生成系统产生O2-的情况显示出对氧浓度的类似依赖性。pH下降导致经酵母聚糖处理的中性粒细胞的耗氧量和O2-产生量均降低，pH 6.0时的值为pH 7.5时观察值的10% - 20%。对无细胞O2-生成系统的实验表明，低pH下呼吸爆发活性的这种下降是由于酸性条件下O2-生成酶的激活效率低下所致。
