Rodríguez-Almonacid Cristian, Fuentes-Rodríguez Gustavo, González Leidy P, Moreno-Torres Carlos, Matta Nubia E
Grupo Caracterización Genética e Inmunología, Departamento de Biología, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia.
Departamento de Salud Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia.
Front Vet Sci. 2022 Sep 15;9:961609. doi: 10.3389/fvets.2022.961609. eCollection 2022.
The Podocnemididae family is seriously affected by anthropogenic factors, which is why almost all of their family members are threatened, according to the IUCN red list. The biology and ecology of these species, as well as the hematological and serum chemistry reference intervals that allow clinical action and decision-making conservation programs, are poorly known. Based on this, the objective of this study was to establish the hematological and blood chemistry parameters of the Savannah side-necked turtle () and Yellow-spotted river turtle () maintained in captivity at the Estación de Biología Tropical Roberto Franco (Villavicencio-Colombia). Forty-nine captive turtles of the species (n = 28) and (n = 21) were sampled to determine hematological and serum chemistry parameters. Blood samples were taken from the jugular veins of both male and female turtles across both species. Student's -test and Mann-Whitney-Wilcoxon tests were used to compare values between the parameters evaluated against genders and sizes. Reference intervals were calculated for the hematological and biochemical values of each species. Some assessed parameters demonstrated significant differences between the males and females of both species. Most of the analyzed parameters exhibited similar reference intervals in both species. In this study, we report values and propose the hematological and serum chemistry reference intervals for and , which can be used in the clinical diagnosis of these reptiles and in future research.
侧颈龟科受到人为因素的严重影响,因此根据国际自然保护联盟红色名录,几乎其所有成员都受到威胁。这些物种的生物学和生态学,以及有助于临床行动和制定保护决策计划的血液学和血清化学参考区间,目前知之甚少。基于此,本研究的目的是确定在热带生物学站罗伯托·佛朗哥站(比亚维森西奥 - 哥伦比亚)圈养的黄斑侧颈龟(Podocnemis expansa)和黄斑河龟(Podocnemis unifilis)的血液学和血液化学参数。对49只圈养的黄斑侧颈龟(n = 28)和黄斑河龟(n = 21)进行采样,以确定血液学和血清化学参数。从两个物种的雄性和雌性乌龟的颈静脉采集血样。使用学生t检验和曼 - 惠特尼 - 威尔科克森检验来比较针对性别和大小评估的参数之间的值。计算了每个物种血液学和生化值的参考区间。一些评估参数在两个物种的雄性和雌性之间表现出显著差异。大多数分析参数在两个物种中表现出相似的参考区间。在本研究中,我们报告了黄斑侧颈龟和黄斑河龟的值,并提出了它们的血液学和血清化学参考区间,可用于这些爬行动物的临床诊断和未来研究。