一种基于离子色谱的便捷方法用于花生仁中植酸的高通量分析。

An expedient ion chromatography based method for high-throughput analysis of phytic acid in groundnut kernels.

作者信息

Verma Aman, Singh Sushmita, Thawait Lokesh K, Mahatma Mahesh K, Singh A L

机构信息

ICAR- Directorate of Groundnut Research, Junagadh, 362001 Gujarat India.

ICAR- Central Arid Zone Research Institute, Jodhpur, 342003 Rajasthan India.

出版信息

J Food Sci Technol. 2022 Nov;59(11):4479-4486. doi: 10.1007/s13197-022-05527-9. Epub 2022 Jun 25.

DOI:10.1007/s13197-022-05527-9

PMID:36193457

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9525535/

Abstract

UNLABELLED

A study was made to expedite ion chromatography method using IonPac analytical column and self-regenerating anion suppressor for phytic acid determination in groundnut seeds and compared with a widely adopted spectrophotometric method based on enzymatic hydrolysis. The Ion Chromatography method equipped with AG11 guard and AS11 analytical columns in isocratic mode using 65 mM NaOH mobile phase at 1 mL min flow rate showed a sharp peak for phytic acid with a retention time of 2.42 ± 0.2 min. The peak area was plotted v/s concentration showed linearity with an R value of 0.997, detection limit of 0.028 mg L and recovery of 98% as against R value of 0.988 and detection limit of 0.065 mg L in the spectrophotometric method. The study demonstrates that Ion Chromatography method was more accurate with a better detection limit than spectrophotometry. Also, this method provides robust handling with lesser reagent requirements due to combined eluent generation and self-regenerating suppression.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13197-022-05527-9.

摘要

未标注

进行了一项研究，以加快使用IonPac分析柱和自再生阴离子抑制器的离子色谱法测定花生种子中的植酸，并与一种广泛采用的基于酶水解的分光光度法进行比较。配备AG11保护柱和AS11分析柱的离子色谱法，在等度模式下使用65 mM NaOH流动相，流速为1 mL/min，植酸呈现出尖锐的峰，保留时间为2.42±0.2分钟。绘制峰面积与浓度的关系图，显示线性关系，R值为0.997，检测限为0.028 mg/L，回收率为98%；而分光光度法的R值为0.988，检测限为0.065 mg/L。该研究表明，离子色谱法比分光光度法更准确，检测限更好。此外，由于采用了组合洗脱液生成和自再生抑制技术，该方法操作简便，试剂需求较少。