[慢性尿毒症患者唾液酸化合物及血液滤过液的分离与核磁共振光谱表征]

[Isolation and NMR spectroscopic characterization of sialic acid compounds and hemofiltrate of chronic uremia patients].

作者信息

Weisshaar G, Baumann W, Friebolin H, Brunner H, Mann H, Sieberth H G, Opferkuch H J

出版信息

Biol Chem Hoppe Seyler. 1987 Jun;368(6):647-58.

PMID:3620108

Abstract

Eight sialyloligosaccharides have been isolated from the hemofiltrate of a patient with end stage renal disease using reverse osmosis, gel filtration, ion-exchange and high-performance liquid chromatography. The structures were predominantly elucidated by one- and two-dimensional 1H- and 13C-NMR spectroscopy: 1 NeuAc alpha 2-3Gal beta 1-4Glc; 2 NeuAc alpha 2-6Gal beta 1-4Glc; 3 NeuAc alpha 2-3Gal beta 1-4GlcNAc; 4 NeuAc-alpha 2-6Gal beta 1-4GlcNAc; 5 NeuAc alpha 2-3Gal beta 1-4-GlcNAc alpha 1-P; 6 NeuAc alpha 2-6Gal beta 1-4GlcNAc alpha 1-P; 7 NeuAc alpha 2-3Gal beta 1-3GalNAc alpha 1-P; 8 NeuAc alpha 2-8NeuAc. While compounds 1-7 are also components of normal human urine, di-N-acetyl-D-neuraminic acid (8) could be isolated for the first time from biological material. The origin and possible clinical relevance of these compounds have to be proved in further investigations.

摘要

利用反渗透、凝胶过滤、离子交换和高效液相色谱法，从一名终末期肾病患者的血液滤过液中分离出了8种唾液酸寡糖。其结构主要通过一维和二维1H-和13C-NMR光谱确定：1 NeuAcα2-3Galβ1-4Glc；2 NeuAcα2-6Galβ1-4Glc；3 NeuAcα2-3Galβ1-4GlcNAc；4 NeuAc-α2-6Galβ1-4GlcNAc；5 NeuAcα2-3Galβ1-4-GlcNAcα1-P；6 NeuAcα2-6Galβ1-4GlcNAcα1-P；7 NeuAcα2-3Galβ1-3GalNAcα1-P；8 NeuAcα2-8NeuAc。虽然化合物1-7也是正常人尿液的成分，但二-N-乙酰-D-神经氨酸（8）首次从生物材料中分离出来。这些化合物的来源及其可能的临床相关性有待进一步研究证实。