Weisshaar G, Baumann W, Friebolin H, Brunner H, Mann H, Sieberth H G, Opferkuch H J
Biol Chem Hoppe Seyler. 1987 Jun;368(6):647-58.
Eight sialyloligosaccharides have been isolated from the hemofiltrate of a patient with end stage renal disease using reverse osmosis, gel filtration, ion-exchange and high-performance liquid chromatography. The structures were predominantly elucidated by one- and two-dimensional 1H- and 13C-NMR spectroscopy: 1 NeuAc alpha 2-3Gal beta 1-4Glc; 2 NeuAc alpha 2-6Gal beta 1-4Glc; 3 NeuAc alpha 2-3Gal beta 1-4GlcNAc; 4 NeuAc-alpha 2-6Gal beta 1-4GlcNAc; 5 NeuAc alpha 2-3Gal beta 1-4-GlcNAc alpha 1-P; 6 NeuAc alpha 2-6Gal beta 1-4GlcNAc alpha 1-P; 7 NeuAc alpha 2-3Gal beta 1-3GalNAc alpha 1-P; 8 NeuAc alpha 2-8NeuAc. While compounds 1-7 are also components of normal human urine, di-N-acetyl-D-neuraminic acid (8) could be isolated for the first time from biological material. The origin and possible clinical relevance of these compounds have to be proved in further investigations.
利用反渗透、凝胶过滤、离子交换和高效液相色谱法，从一名终末期肾病患者的血液滤过液中分离出了8种唾液酸寡糖。其结构主要通过一维和二维1H-和13C-NMR光谱确定：1 NeuAcα2-3Galβ1-4Glc；2 NeuAcα2-6Galβ1-4Glc；3 NeuAcα2-3Galβ1-4GlcNAc；4 NeuAc-α2-6Galβ1-4GlcNAc；5 NeuAcα2-3Galβ1-4-GlcNAcα1-P；6 NeuAcα2-6Galβ1-4GlcNAcα1-P；7 NeuAcα2-3Galβ1-3GalNAcα1-P；8 NeuAcα2-8NeuAc。虽然化合物1-7也是正常人尿液的成分，但二-N-乙酰-D-神经氨酸（8）首次从生物材料中分离出来。这些化合物的来源及其可能的临床相关性有待进一步研究证实。
